MICROSATELLITE POLYMORPHISMS FOR CHROMOSOME-5 BANDS Q11.2-Q13.3

R SHERRINGTON; B MANKOO; M DIXON; D CURTIS; G KALSI; G MELMER; H GURLING

doi:10.1159/000154177

MICROSATELLITE POLYMORPHISMS FOR CHROMOSOME-5 BANDS Q11.2-Q13.3

R SHERRINGTON, B MANKOO, M DIXON, D CURTIS, G KALSI, G MELMER, H GURLING

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11 Citations (Scopus)

Abstract

The genes for spinal muscular atrophy (SMA) and a possible subtype of schizophrenia (SCZD1) have been mapped to chromosome 5q11.2-q13.3. DNA markers have been mapped to 5q11.2-q13.3 using a hybrid cell line deleted for this region [Gilliam et al., Genomics 1989;5:940-944]. Genomic lambda clones for these markers facilitated the identification of highly polymorphic microsatellites. A total of ten microsatellites were identified and sequenced. Of these, seven were found to be polymorphic. Four had polymorphism information content values > 0.7. New polymorphic microsatellites were sequenced for D5S76, D5S125, D5S39, D5S127 and HEX-B. Two-point and multipoint analysis in non-CEPH pedigrees confirmed that the microsatellites were in tight linkage with each other. These new microsatellites will increase the efficiency of linkage analysis for these disorders.

Original language	English
Pages (from-to)	197-202
Number of pages	6
Journal	Human Heredity
Volume	43
Issue number	3
DOIs	https://doi.org/10.1159/000154177
Publication status	Published - 1993

Keywords

LOCUS
GENE
SPINAL MUSCULAR ATROPHY
GENETICS
SPINAL MUSCULAR-ATROPHY
POLYMORPHISM, MICROSATELLITE
CHROMOSOME-5
SCHIZOPHRENIA
LENGTH POLYMORPHISMS

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title = "MICROSATELLITE POLYMORPHISMS FOR CHROMOSOME-5 BANDS Q11.2-Q13.3",

abstract = "The genes for spinal muscular atrophy (SMA) and a possible subtype of schizophrenia (SCZD1) have been mapped to chromosome 5q11.2-q13.3. DNA markers have been mapped to 5q11.2-q13.3 using a hybrid cell line deleted for this region [Gilliam et al., Genomics 1989;5:940-944]. Genomic lambda clones for these markers facilitated the identification of highly polymorphic microsatellites. A total of ten microsatellites were identified and sequenced. Of these, seven were found to be polymorphic. Four had polymorphism information content values > 0.7. New polymorphic microsatellites were sequenced for D5S76, D5S125, D5S39, D5S127 and HEX-B. Two-point and multipoint analysis in non-CEPH pedigrees confirmed that the microsatellites were in tight linkage with each other. These new microsatellites will increase the efficiency of linkage analysis for these disorders.",

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author = "R SHERRINGTON and B MANKOO and M DIXON and D CURTIS and G KALSI and G MELMER and H GURLING",

year = "1993",

doi = "10.1159/000154177",

language = "English",

volume = "43",

pages = "197--202",

journal = "Human Heredity",

issn = "0001-5652",

publisher = "S. Karger AG",

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TY - JOUR

T1 - MICROSATELLITE POLYMORPHISMS FOR CHROMOSOME-5 BANDS Q11.2-Q13.3

AU - SHERRINGTON, R

AU - MANKOO, B

AU - DIXON, M

AU - CURTIS, D

AU - KALSI, G

AU - MELMER, G

AU - GURLING, H

PY - 1993

Y1 - 1993

N2 - The genes for spinal muscular atrophy (SMA) and a possible subtype of schizophrenia (SCZD1) have been mapped to chromosome 5q11.2-q13.3. DNA markers have been mapped to 5q11.2-q13.3 using a hybrid cell line deleted for this region [Gilliam et al., Genomics 1989;5:940-944]. Genomic lambda clones for these markers facilitated the identification of highly polymorphic microsatellites. A total of ten microsatellites were identified and sequenced. Of these, seven were found to be polymorphic. Four had polymorphism information content values > 0.7. New polymorphic microsatellites were sequenced for D5S76, D5S125, D5S39, D5S127 and HEX-B. Two-point and multipoint analysis in non-CEPH pedigrees confirmed that the microsatellites were in tight linkage with each other. These new microsatellites will increase the efficiency of linkage analysis for these disorders.

AB - The genes for spinal muscular atrophy (SMA) and a possible subtype of schizophrenia (SCZD1) have been mapped to chromosome 5q11.2-q13.3. DNA markers have been mapped to 5q11.2-q13.3 using a hybrid cell line deleted for this region [Gilliam et al., Genomics 1989;5:940-944]. Genomic lambda clones for these markers facilitated the identification of highly polymorphic microsatellites. A total of ten microsatellites were identified and sequenced. Of these, seven were found to be polymorphic. Four had polymorphism information content values > 0.7. New polymorphic microsatellites were sequenced for D5S76, D5S125, D5S39, D5S127 and HEX-B. Two-point and multipoint analysis in non-CEPH pedigrees confirmed that the microsatellites were in tight linkage with each other. These new microsatellites will increase the efficiency of linkage analysis for these disorders.

KW - LOCUS

KW - GENE

KW - SPINAL MUSCULAR ATROPHY

KW - GENETICS

KW - SPINAL MUSCULAR-ATROPHY

KW - POLYMORPHISM, MICROSATELLITE

KW - CHROMOSOME-5

KW - SCHIZOPHRENIA

KW - LENGTH POLYMORPHISMS

U2 - 10.1159/000154177

DO - 10.1159/000154177

M3 - Article

SN - 0001-5652

VL - 43

SP - 197

EP - 202

JO - Human Heredity

JF - Human Heredity

IS - 3

ER -

MICROSATELLITE POLYMORPHISMS FOR CHROMOSOME-5 BANDS Q11.2-Q13.3

Abstract

Keywords

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