Microwave-assisted synthesis of [52Mn]Mn-porphyrins: Applications in cell and liposome radiolabelling

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
74 Downloads (Pure)

Abstract

Background: Manganese porphyrins have several therapeutic/imaging applications, including their use as radioprotectants (in clinical trials) and as paramagnetic MRI contrast agents. The affinity of porphyrins for lipid bilayers also makes them candidates for cell/liposome labelling. We hypothesised that metalation with the positron emission tomography (PET) radionuclide 52Mn (t1/2 = 5.6 d) would allow long-term in vivo biodistribution studies of Mn-porphyrins, as well as a method to label and track cells/liposomes, but methods for fast and efficient radiolabelling are lacking.
Results: Several porphyrins were produced and radiolabelled by addition to neutralised [52Mn]MnCl2 and heat- ing using a microwave (MW) synthesiser, and compared with non-MW heating. MW radiosynthesis allowed > 95 % radiochemical yields (RCY) in just 1 h. Conversely, non-MW heating at 70 °C for 1 h resulted in low RCY (0–25 % RCY) and most porphyrins did not reach radiolabelling completion after 24 h. Formation of the 52Mn-complexes were confirmed with radio-HPLC by comparison with their non-radioactive 55Mn counterparts. Following this, several [52Mn]Mn-porphyrins were used to radiolabel liposomes resulting in 75–86 % la- belling efficiency (LE). Two lead [52Mn]Mn-porphyrins were taken forward to label MDA-MB-231 cancer cells in vitro, achieving ca. 11 % LE. After 24 h, 32–45 % of the [52Mn]Mn-porphyrins was retained in cells.

Conclusions: In contrast to standard methods, MW heating allows the fast synthesis of [52Mn]Mn-porphyrins with >95 % radiochemical yields that avoid purification. [52Mn]Mn-porphyrins also show promising cell/liposome labelling properties. Our reported technique can potentially be exploited for the in vivo imaging of Mn-porphyrin therapeutics, as well as for the accurate in vivo quantification of Mn-porphyrin MRI agents.
Original languageEnglish
Pages (from-to)6-17
Number of pages12
JournalNuclear Medicine and Biology
Volume114-115
Early online date8 Sept 2022
DOIs
Publication statusPublished - Nov 2022

Fingerprint

Dive into the research topics of 'Microwave-assisted synthesis of [52Mn]Mn-porphyrins: Applications in cell and liposome radiolabelling'. Together they form a unique fingerprint.

Cite this