Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C

Javier Casares-Arias; María Ujué González; Alvaro San Paulo; Leandro N. Ventimiglia; Jessica B.A. Sadler; David G. Miguez; Leticia Labat-de-Hoz; Armando Rubio-Ramos; Laura Rangel; Miguel Bernabé-Rubio; Jaime Fernández-Barrera; Isabel Correas; Juan Martín-Serrano; Miguel A. Alonso

doi:10.1016/j.isci.2020.101244

Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C

Javier Casares-Arias, María Ujué González, Alvaro San Paulo, Leandro N. Ventimiglia, Jessica B.A. Sadler, David G. Miguez, Leticia Labat-de-Hoz, Armando Rubio-Ramos, Laura Rangel, Miguel Bernabé-Rubio, Jaime Fernández-Barrera, Isabel Correas, Juan Martín-Serrano, Miguel A. Alonso^*

^*Corresponding author for this work

Infectious Diseases

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The inheritance of the midbody remnant (MBR) breaks the symmetry of the two daughter cells, with functional consequences for lumen and primary cilium formation by polarized epithelial cells, and also for development and differentiation. However, despite its importance, neither the relationship between the plasma membrane and the inherited MBR nor the mechanism of MBR inheritance is well known. Here, the analysis by correlative light and ultra-high-resolution scanning electron microscopy reveals a membranous stalk that physically connects the MBR to the apical membrane of epithelial cells. The stalk, which derives from the uncleaved side of the midbody, concentrates the ESCRT machinery. The ESCRT CHMP4C subunit enables MBR inheritance, and its depletion dramatically reduces the percentage of ciliated cells. We demonstrate (1) that MBRs are physically connected to the plasma membrane, (2) how CHMP4C helps maintain the integrity of the connection, and (3) the functional importance of the connection.

Original language	English
Article number	101244
Journal	iScience
Volume	23
Issue number	6
DOIs	https://doi.org/10.1016/j.isci.2020.101244
Publication status	Published - 26 Jun 2020

Keywords

Cell Biology
Immunology
Neuroscience

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Casares-Arias, J., González, M. U., San Paulo, A., Ventimiglia, L. N., Sadler, J. B. A., Miguez, D. G., Labat-de-Hoz, L., Rubio-Ramos, A., Rangel, L., Bernabé-Rubio, M., Fernández-Barrera, J., Correas, I., Martín-Serrano, J., & Alonso, M. A. (2020). Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C. iScience, 23(6), [101244]. https://doi.org/10.1016/j.isci.2020.101244

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title = "Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C",

abstract = "The inheritance of the midbody remnant (MBR) breaks the symmetry of the two daughter cells, with functional consequences for lumen and primary cilium formation by polarized epithelial cells, and also for development and differentiation. However, despite its importance, neither the relationship between the plasma membrane and the inherited MBR nor the mechanism of MBR inheritance is well known. Here, the analysis by correlative light and ultra-high-resolution scanning electron microscopy reveals a membranous stalk that physically connects the MBR to the apical membrane of epithelial cells. The stalk, which derives from the uncleaved side of the midbody, concentrates the ESCRT machinery. The ESCRT CHMP4C subunit enables MBR inheritance, and its depletion dramatically reduces the percentage of ciliated cells. We demonstrate (1) that MBRs are physically connected to the plasma membrane, (2) how CHMP4C helps maintain the integrity of the connection, and (3) the functional importance of the connection.",

keywords = "Cell Biology, Immunology, Neuroscience",

author = "Javier Casares-Arias and Gonz{\'a}lez, {Mar{\'i}a Uju{\'e}} and {San Paulo}, Alvaro and Ventimiglia, {Leandro N.} and Sadler, {Jessica B.A.} and Miguez, {David G.} and Leticia Labat-de-Hoz and Armando Rubio-Ramos and Laura Rangel and Miguel Bernab{\'e}-Rubio and Jaime Fern{\'a}ndez-Barrera and Isabel Correas and Juan Mart{\'i}n-Serrano and Alonso, {Miguel A.}",

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Casares-Arias, J, González, MU, San Paulo, A, Ventimiglia, LN , Sadler, JBA, Miguez, DG, Labat-de-Hoz, L, Rubio-Ramos, A, Rangel, L, Bernabé-Rubio, M, Fernández-Barrera, J, Correas, I, Martín-Serrano, J & Alonso, MA 2020, 'Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C', iScience, vol. 23, no. 6, 101244. https://doi.org/10.1016/j.isci.2020.101244

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AU - Casares-Arias, Javier

AU - González, María Ujué

AU - San Paulo, Alvaro

AU - Ventimiglia, Leandro N.

AU - Sadler, Jessica B.A.

AU - Miguez, David G.

AU - Labat-de-Hoz, Leticia

AU - Rubio-Ramos, Armando

AU - Rangel, Laura

AU - Bernabé-Rubio, Miguel

AU - Fernández-Barrera, Jaime

AU - Correas, Isabel

AU - Martín-Serrano, Juan

AU - Alonso, Miguel A.

PY - 2020/6/26

Y1 - 2020/6/26

N2 - The inheritance of the midbody remnant (MBR) breaks the symmetry of the two daughter cells, with functional consequences for lumen and primary cilium formation by polarized epithelial cells, and also for development and differentiation. However, despite its importance, neither the relationship between the plasma membrane and the inherited MBR nor the mechanism of MBR inheritance is well known. Here, the analysis by correlative light and ultra-high-resolution scanning electron microscopy reveals a membranous stalk that physically connects the MBR to the apical membrane of epithelial cells. The stalk, which derives from the uncleaved side of the midbody, concentrates the ESCRT machinery. The ESCRT CHMP4C subunit enables MBR inheritance, and its depletion dramatically reduces the percentage of ciliated cells. We demonstrate (1) that MBRs are physically connected to the plasma membrane, (2) how CHMP4C helps maintain the integrity of the connection, and (3) the functional importance of the connection.

AB - The inheritance of the midbody remnant (MBR) breaks the symmetry of the two daughter cells, with functional consequences for lumen and primary cilium formation by polarized epithelial cells, and also for development and differentiation. However, despite its importance, neither the relationship between the plasma membrane and the inherited MBR nor the mechanism of MBR inheritance is well known. Here, the analysis by correlative light and ultra-high-resolution scanning electron microscopy reveals a membranous stalk that physically connects the MBR to the apical membrane of epithelial cells. The stalk, which derives from the uncleaved side of the midbody, concentrates the ESCRT machinery. The ESCRT CHMP4C subunit enables MBR inheritance, and its depletion dramatically reduces the percentage of ciliated cells. We demonstrate (1) that MBRs are physically connected to the plasma membrane, (2) how CHMP4C helps maintain the integrity of the connection, and (3) the functional importance of the connection.

KW - Cell Biology

KW - Immunology

KW - Neuroscience

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VL - 23

JO - iScience

JF - iScience

IS - 6

M1 - 101244

ER -

Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C

Abstract

Keywords

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