@techreport{af41d3b462c944f2a49093ce18bd30a9,
title = "Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity",
abstract = "Antimicrobial peptides (AMPs) are a potential source of new molecules to counter the increase in antimicrobial resistant infections but a better understanding of their properties is required for effective translation as therapeutics. Details of the mechanism of their interaction with the bacterial plasma membrane are desired since damage or penetration of this structure is considered essential for AMP activity. Relatively modest modifications to AMP primary sequence can induce substantial changes in potency and/or spectrum of activity but, hitherto, have not been predicted to substantially alter the mechanism of interaction with the bacterial plasma membrane. Here we use a combination of molecular dynamics simulations, circular dichroism, liquid- and solid-state NMR and patch clamp to investigate the extent to which temporin B and its analogues can be distinguished both in vitro and in silico on the basis of their interactions with model membranes. Enhancing the hydrophobicity of the N-terminus and cationicity of the C-terminus in temporin B improves its membrane activity and potency against both Gram-negative and Gram-positive bacteria. In contrast, enhancing the cationicity of the N-terminus abrogates its ability to trigger channel conductance and renders it ineffective against Staphylococcus aureus while nevertheless enhancing its potency against Escherichia coli. Our findings suggest even closely related AMPs may target the same bacterium with fundamentally differing mechanisms of action.",
author = "Giorgia Manzo and Ferguson, {Philip Mark} and Gustilo, {V Benjamin} and Charlotte Hind and Melanie Clifford and Tam Bui and Alexander Drake and Atkinson, {Robert Andrew} and Sutton, {J. Mark} and Giovanna Batoni and Christian Lorenz and David Phoenix and Mason, {Andrew James}",
year = "2018",
month = may,
day = "1",
doi = "10.1101/312215",
language = "English",
series = "bioRxiv",
publisher = "Cold Spring Harbor Labs Journals",
type = "WorkingPaper",
institution = "Cold Spring Harbor Labs Journals",
}