miR-195 inhibits tumor growth and angiogenesis through modulating IRS1 in breast cancer

TY - JOUR

T1 - miR-195 inhibits tumor growth and angiogenesis through modulating IRS1 in breast cancer

AU - Wang, Yilin

AU - Zhang, Xiaolong

AU - Zou, Chao

AU - Kung, Hsiang-Fu

AU - Lin, Marie C.

AU - Dress, Andreas

AU - Wardle, Fiona

AU - Jiang, Bing-Hua

AU - Lai, Lihui

PY - 2016/5

Y1 - 2016/5

N2 - Angiogenesis has been found as an attractive target for drug therapy as it is necessary for tumor growth. Accumulating evidences show that microRNAs (miRNAs), which are a group of highly conserved, single-stranded, short non-coding RNAs, play important roles through directly targeting angiogenic factors and protein kinases. The purpose of this study is to investigate the role of miR-195 in breast cancer development and angiogenesis through targeting IRS1. We show that miR-195 is inversely related with Insulin receptor substrate 1 (IRS1) in both breast cancer cells and breast cancer tissues. Induction of miR-195 could suppress IRS1 protein expression through binding to its 3′UTR regions either by transfection with miR-195 oligo or by infection with lentivirus encoding miR-195 gene. Moreover, re-expression of IRS1 reverses miR-195-mediated repression of tumor cell growth and miR-195 inhibits tumor angiogenesis through suppressing IRS1-VEGF axis. These data suggest that miR-195 mimics are potential therapeutic agents for breast cancer diagnose.

AB - Angiogenesis has been found as an attractive target for drug therapy as it is necessary for tumor growth. Accumulating evidences show that microRNAs (miRNAs), which are a group of highly conserved, single-stranded, short non-coding RNAs, play important roles through directly targeting angiogenic factors and protein kinases. The purpose of this study is to investigate the role of miR-195 in breast cancer development and angiogenesis through targeting IRS1. We show that miR-195 is inversely related with Insulin receptor substrate 1 (IRS1) in both breast cancer cells and breast cancer tissues. Induction of miR-195 could suppress IRS1 protein expression through binding to its 3′UTR regions either by transfection with miR-195 oligo or by infection with lentivirus encoding miR-195 gene. Moreover, re-expression of IRS1 reverses miR-195-mediated repression of tumor cell growth and miR-195 inhibits tumor angiogenesis through suppressing IRS1-VEGF axis. These data suggest that miR-195 mimics are potential therapeutic agents for breast cancer diagnose.

KW - MiR-195

KW - Insulin receptor substrate 1

KW - Breast cancer

KW - Angiogenesis

U2 - 10.1016/j.biopha.2016.03.007

DO - 10.1016/j.biopha.2016.03.007

M3 - Article

SN - 0753-3322

VL - 80

SP - 95

EP - 101

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

ER -