TY - JOUR
T1 - Modeling the human bone marrow niche in mice
T2 - From host bone marrow engraftment to bioengineering approaches
AU - Abarrategi, Ander
AU - Mian, Syed A
AU - Passaro, Diana
AU - Rouault-Pierre, Kevin
AU - Grey, William
AU - Bonnet, Dominique
N1 - © 2018 Abarrategi et al.
PY - 2018/3/5
Y1 - 2018/3/5
N2 - Xenotransplantation of patient-derived samples in mouse models has been instrumental in depicting the role of hematopoietic stem and progenitor cells in the establishment as well as progression of hematological malignancies. The foundations for this field of research have been based on the development of immunodeficient mouse models, which provide normal and malignant human hematopoietic cells with a supportive microenvironment. Immunosuppressed and genetically modified mice expressing human growth factors were key milestones in patient-derived xenograft (PDX) models, highlighting the importance of developing humanized microenvironments. The latest major improvement has been the use of human bone marrow (BM) niche-forming cells to generate human-mouse chimeric BM tissues in PDXs, which can shed light on the interactions between human stroma and hematopoietic cells. Here, we summarize the methods used for human hematopoietic cell xenotransplantation and their milestones and review the latest approaches in generating humanized BM tissues in mice to study human normal and malignant hematopoiesis.
AB - Xenotransplantation of patient-derived samples in mouse models has been instrumental in depicting the role of hematopoietic stem and progenitor cells in the establishment as well as progression of hematological malignancies. The foundations for this field of research have been based on the development of immunodeficient mouse models, which provide normal and malignant human hematopoietic cells with a supportive microenvironment. Immunosuppressed and genetically modified mice expressing human growth factors were key milestones in patient-derived xenograft (PDX) models, highlighting the importance of developing humanized microenvironments. The latest major improvement has been the use of human bone marrow (BM) niche-forming cells to generate human-mouse chimeric BM tissues in PDXs, which can shed light on the interactions between human stroma and hematopoietic cells. Here, we summarize the methods used for human hematopoietic cell xenotransplantation and their milestones and review the latest approaches in generating humanized BM tissues in mice to study human normal and malignant hematopoiesis.
U2 - 10.1084/jem.20172139
DO - 10.1084/jem.20172139
M3 - Review article
C2 - 29453226
SN - 0022-1007
VL - 215
SP - 729
EP - 743
JO - The Journal of experimental medicine
JF - The Journal of experimental medicine
IS - 3
ER -
