Modeling the human bone marrow niche in mice: From host bone marrow engraftment to bioengineering approaches

Ander Abarrategi, Syed A Mian, Diana Passaro, Kevin Rouault-Pierre, William Grey, Dominique Bonnet

    Research output: Contribution to journalReview articlepeer-review

    86 Citations (Scopus)

    Abstract

    Xenotransplantation of patient-derived samples in mouse models has been instrumental in depicting the role of hematopoietic stem and progenitor cells in the establishment as well as progression of hematological malignancies. The foundations for this field of research have been based on the development of immunodeficient mouse models, which provide normal and malignant human hematopoietic cells with a supportive microenvironment. Immunosuppressed and genetically modified mice expressing human growth factors were key milestones in patient-derived xenograft (PDX) models, highlighting the importance of developing humanized microenvironments. The latest major improvement has been the use of human bone marrow (BM) niche-forming cells to generate human-mouse chimeric BM tissues in PDXs, which can shed light on the interactions between human stroma and hematopoietic cells. Here, we summarize the methods used for human hematopoietic cell xenotransplantation and their milestones and review the latest approaches in generating humanized BM tissues in mice to study human normal and malignant hematopoiesis.

    Original languageEnglish
    Pages (from-to)729-743
    Number of pages15
    JournalThe Journal of experimental medicine
    Volume215
    Issue number3
    DOIs
    Publication statusPublished - 5 Mar 2018

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