Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule

Gabriella Honeth; Tommaso Schiavinotto; Federico Vaggi; Rebecca Marlow; Tokuwa Kanno; Ireneusz Shinomiya; Sara Lombardi; Bharath Buchupalli; Rosalind Graham; Patrycja Gazinska; Vernie Ramalingam; Joy Burchell; Anand D Purushotham; Sarah E Pinder; Attila Csikasz-Nagy; Gabriela Dontu

doi:10.1016/j.stemcr.2015.02.013

Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule

Gabriella Honeth, Tommaso Schiavinotto, Federico Vaggi, Rebecca Marlow, Tokuwa Kanno, Ireneusz Shinomiya, Sara Lombardi, Bharath Buchupalli, Rosalind Graham, Patrycja Gazinska, Vernie Ramalingam, Joy Burchell, Anand D Purushotham, Sarah E Pinder, Attila Csikasz-Nagy, Gabriela Dontu

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

222 Downloads (Pure)

Abstract

Characterization of normal breast stem cells is important for understanding their role in breast development and in breast cancer. However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known. In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals. Comparing predictions of these models with immunohistochemical analysis of tissue sections for candidate stem cell markers, we defined distinct areas where stem cells reside in the mammary lobule. An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of nulliparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.

Original language	English
Pages (from-to)	699-711
Number of pages	13
Journal	Stem Cell Reports
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/j.stemcr.2015.02.013
Publication status	Published - 14 Apr 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.stemcr.2015.02.013

1_s2.0_S2213671115000697_mainFinal published version, 3.44 MBLicence: CC BY-NC-ND

Cite this

Honeth, G., Schiavinotto, T., Vaggi, F., Marlow, R., Kanno, T., Shinomiya, I., Lombardi, S., Buchupalli, B., Graham, R., Gazinska, P., Ramalingam, V., Burchell, J., Purushotham, A. D., Pinder, S. E., Csikasz-Nagy, A., & Dontu, G. (2015). Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule. Stem Cell Reports, 4(4), 699-711. https://doi.org/10.1016/j.stemcr.2015.02.013

@article{bc6452bfda9f4d228a48156f0cb4610c,

title = "Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule",

abstract = "Characterization of normal breast stem cells is important for understanding their role in breast development and in breast cancer. However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known. In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals. Comparing predictions of these models with immunohistochemical analysis of tissue sections for candidate stem cell markers, we defined distinct areas where stem cells reside in the mammary lobule. An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of nulliparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.",

author = "Gabriella Honeth and Tommaso Schiavinotto and Federico Vaggi and Rebecca Marlow and Tokuwa Kanno and Ireneusz Shinomiya and Sara Lombardi and Bharath Buchupalli and Rosalind Graham and Patrycja Gazinska and Vernie Ramalingam and Joy Burchell and Purushotham, {Anand D} and Pinder, {Sarah E} and Attila Csikasz-Nagy and Gabriela Dontu",

year = "2015",

month = apr,

day = "14",

doi = "10.1016/j.stemcr.2015.02.013",

language = "English",

volume = "4",

pages = "699--711",

journal = "Stem Cell Reports",

issn = "2213-6711",

publisher = "CELL PRESS",

number = "4",

}

Honeth, G, Schiavinotto, T, Vaggi, F, Marlow, R , Kanno, T, Shinomiya, I, Lombardi, S, Buchupalli, B, Graham, R, Gazinska, P, Ramalingam, V, Burchell, J , Purushotham, AD , Pinder, SE , Csikasz-Nagy, A & Dontu, G 2015, 'Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule', Stem Cell Reports, vol. 4, no. 4, pp. 699-711. https://doi.org/10.1016/j.stemcr.2015.02.013

TY - JOUR

T1 - Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule

AU - Honeth, Gabriella

AU - Schiavinotto, Tommaso

AU - Vaggi, Federico

AU - Marlow, Rebecca

AU - Kanno, Tokuwa

AU - Shinomiya, Ireneusz

AU - Lombardi, Sara

AU - Buchupalli, Bharath

AU - Graham, Rosalind

AU - Gazinska, Patrycja

AU - Ramalingam, Vernie

AU - Burchell, Joy

AU - Purushotham, Anand D

AU - Pinder, Sarah E

AU - Csikasz-Nagy, Attila

AU - Dontu, Gabriela

PY - 2015/4/14

Y1 - 2015/4/14

N2 - Characterization of normal breast stem cells is important for understanding their role in breast development and in breast cancer. However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known. In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals. Comparing predictions of these models with immunohistochemical analysis of tissue sections for candidate stem cell markers, we defined distinct areas where stem cells reside in the mammary lobule. An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of nulliparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.

AB - Characterization of normal breast stem cells is important for understanding their role in breast development and in breast cancer. However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known. In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals. Comparing predictions of these models with immunohistochemical analysis of tissue sections for candidate stem cell markers, we defined distinct areas where stem cells reside in the mammary lobule. An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of nulliparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.

U2 - 10.1016/j.stemcr.2015.02.013

DO - 10.1016/j.stemcr.2015.02.013

M3 - Article

C2 - 25818813

SN - 2213-6711

VL - 4

SP - 699

EP - 711

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 4

ER -

Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this