Modulation of dipalmitoylphosphatidylcholine monolayers by dimethyl sulphoxide

Aleksandra P Dabkowska, Louise E Collins, David J Barlow, Robert David Barker, Sylvia E McLain, M Jayne Lawrence, Christian D Lorenz

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


The action of the penetration-enhancing agent, dimethylsulphoxide (DMSO), on phospholipid monolayers was investigated at the air-water interface using a combination of experimental techniques and molecular dynamics simulations. Brewster angle microscopy revealed that DPPC monolayers remained laterally homogenous at sub-phase concentrations up to a mole fraction of 0.1 DMSO. Neutron reflectometry of the monolayers in combination with isotopic substitution enabled the determination of solvent profiles as a function of distance perpendicular to the interface for the different DMSO sub-phase concentrations. These experimental results were compared to those obtained from molecular dynamic (MD) simulations of the corresponding monolayer systems. There was excellent agreement found between the MD-derived reflectivity curves and the measured data for all of the H/D contrast variations investigated. The MD provide a detailed description of the distribution of water and DMSO molecules around the phosphatidylcholine head group, and how this distribution changes with increasing DMSO concentrations. Significantly, the measurements and simulations that are reported here support the hypothesis that DMSO acts by dehydrating the phosphatidylcholine head group, and as such provide the first direct evidence that it does so primarily by displacing water molecules bound to the choline group.
Original languageEnglish
Publication statusE-pub ahead of print - 2014


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