Molecular magnetic resonance imaging of atrial clots in a swine model

TY - JOUR

T1 - Molecular magnetic resonance imaging of atrial clots in a swine model

AU - Spuentrup, E

AU - Fausten, B

AU - Kinzel, S

AU - Wiethoff, A J

AU - Botnar, R M

AU - Graham, P B

AU - Haller, S

AU - Katoh, M

AU - Parsons, E C

AU - Manning, W J

AU - Busch, T

AU - Gunther, R W

AU - Buecker, A

PY - 2005/7/19

Y1 - 2005/7/19

N2 - Background - The detection and differentiation of intracardiac masses is still challenging and may include neoplasms and thrombi. The aim of this study was the investigation of a targeted, fibrin-specific contrast agent (EP-2104R) for molecular targeted magnetic resonance imaging (MRI) of left atrial clots. Methods and Results - Chronic human thrombi were surgically implanted in the left atrial appendage of 5 swine. Molecular MRI was performed with a navigator-gated, free-breathing, cardiac-triggered 3D inversion-recovery, black-blood, gradient-echo sequence before and after systemic administration of 4 mu mol/kg EP-2104R. MR images were analyzed by 2 investigators, and the contrast-to-noise ratio was calculated. Location of clots was confirmed by autopsy, and the gadolinium concentration in the clots was assessed. Before contrast agent administration, thrombi were not visible on black-blood MR images. After contrast administration, all atrial clots ( n = 5) were selectively visualized as white spots with a high contrast-to-noise ratio (clot/blood, 29.7 +/- 8.0). The gadolinium concentration in the clots averaged 74 +/- 45 mu mol/L. Conclusions - The fibrin-specific MR contrast agent EP-2104R allows for selective and high-contrast visualization of left atrial clots by means of molecular targeted MRI

AB - Background - The detection and differentiation of intracardiac masses is still challenging and may include neoplasms and thrombi. The aim of this study was the investigation of a targeted, fibrin-specific contrast agent (EP-2104R) for molecular targeted magnetic resonance imaging (MRI) of left atrial clots. Methods and Results - Chronic human thrombi were surgically implanted in the left atrial appendage of 5 swine. Molecular MRI was performed with a navigator-gated, free-breathing, cardiac-triggered 3D inversion-recovery, black-blood, gradient-echo sequence before and after systemic administration of 4 mu mol/kg EP-2104R. MR images were analyzed by 2 investigators, and the contrast-to-noise ratio was calculated. Location of clots was confirmed by autopsy, and the gadolinium concentration in the clots was assessed. Before contrast agent administration, thrombi were not visible on black-blood MR images. After contrast administration, all atrial clots ( n = 5) were selectively visualized as white spots with a high contrast-to-noise ratio (clot/blood, 29.7 +/- 8.0). The gadolinium concentration in the clots averaged 74 +/- 45 mu mol/L. Conclusions - The fibrin-specific MR contrast agent EP-2104R allows for selective and high-contrast visualization of left atrial clots by means of molecular targeted MRI

U2 - 10.1161/CIRCULATIONAHA.104.529941

DO - 10.1161/CIRCULATIONAHA.104.529941

M3 - Article

SN - 1524-4539

VL - 112

SP - 396

EP - 399

JO - Circulation (Baltimore)

JF - Circulation (Baltimore)

IS - 3

ER -