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Monitoring Allogeneic CAR-T Cells Using Flow Cytometry

Research output: Contribution to journalArticle

Agnieszka Jozwik, Alan Dunlop, Katy Sanchez, Reuben Benjamin

Original languageEnglish
Pages (from-to)293-308
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume2097
DOIs
Published1 Jan 2020

King's Authors

Abstract

Chimeric antigen receptor T cell (CAR-T) therapies have now entered mainstream clinical practice with two approved autologous CAR-T products targeting CD19 and numerous other products in early and late phase clinical trials. This has led to a demand for highly sensitive, specific, and easily reproducible methods to monitor CAR-T cells in patients. Here we describe a flow cytometry based protocol for detection of allogeneic CAR-T cells and for monitoring their phenotype and numbers in blood and bone marrow of patients following CAR-T treatment.

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