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Monitoring Coronavirus Disease 2019: A Review of Available Diagnostic Tools

Research output: Contribution to journalReview articlepeer-review

Shanshan Liu, Qiuyue Li, Xuntao Chu, Minxia Zeng, Mingbin Liu, Xiaomeng He, Heng Zou, Jianghua Zheng, Christopher Corpe, Xiaoyan Zhang, Jianqing Xu, Jin Wang

Original languageEnglish
Article number672215
Pages (from-to)672215
JournalFrontiers in Public Health
Published7 Jun 2021

Bibliographical note

Funding Information: This research was supported by a grant from the Science and Technology Commission of Shanghai (20Y11900700), a grant from the Special Research Fund of Youan Medical Alliance for the Liver and Infectious Diseases (LM202020), a grant from the National Special Research Program of China for Important Infectious Diseases (2018ZX10302103-003), and a grant from the National Natural Science Foundation of China (81672383). Publisher Copyright: © Copyright © 2021 Liu, Li, Chu, Zeng, Liu, He, Zou, Zheng, Corpe, Zhang, Xu and Wang. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

King's Authors


Coronavirus disease 2019 (COVID-19) pneumonia is caused by the virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has rapidly become a global public health concern. As the new type of betacoronavirus, SARS-CoV-2 can spread across species and between populations and has a greater risk of transmission than other coronaviruses. To control the spread of SARS-CoV-2, it is vital to have a rapid and effective means of diagnosing asymptomatic SARS-CoV-2-positive individuals and patients with COVID-19, an early isolation protocol for infected individuals, and effective treatments for patients with COVID-19 pneumonia. In this review, we will summarize the novel diagnostic tools that are currently available for coronavirus, including imaging examinations and laboratory medicine by next-generation sequencing (NGS), real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) analysis, immunoassay for COVID-19, cytokine and T cell immunoassays, biochemistry and microbiology laboratory parameters in the blood of the patients with COVID-19, and a field-effect transistor-based biosensor of COVID-19. Specifically, we will discuss the effective detection rate and assay time for the rRT-PCR analysis of SARS-CoV-2 and the sensitivity and specificity of different antibody detection methods, such as colloidal gold and ELISA using specimen sources obtained from the respiratory tract, peripheral serum or plasma, and other bodily fluids. Such diagnostics will help scientists and clinicians develop appropriate strategies to combat COVID-19.

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