Monitoring the Growth of an Orthotopic Tumour Xenograft Model: Multi-Modal Imaging Assessment with Benchtop MRI (1T), High-Field MRI (9.4T), Ultrasound and Bioluminescence

TY - JOUR

T1 - Monitoring the Growth of an Orthotopic Tumour Xenograft Model

T2 - Multi-Modal Imaging Assessment with Benchtop MRI (1T), High-Field MRI (9.4T), Ultrasound and Bioluminescence

AU - Ramasawmy, Rajiv

AU - Johnson, S Peter

AU - Roberts, Thomas A

AU - Stuckey, Daniel J

AU - David, Anna L

AU - Pedley, R Barbara

AU - Lythgoe, Mark F

AU - Siow, Bernard

AU - Walker-Samuel, Simon

PY - 2016

Y1 - 2016

N2 - BACKGROUND: Research using orthotopic and transgenic models of cancer requires imaging methods to non-invasively quantify tumour burden. As the choice of appropriate imaging modality is wide-ranging, this study aimed to compare low-field (1T) magnetic resonance imaging (MRI), a novel and relatively low-cost system, against established preclinical techniques: bioluminescence imaging (BLI), ultrasound imaging (US), and high-field (9.4T) MRI.METHODS: A model of colorectal metastasis to the liver was established in eight mice, which were imaged with each modality over four weeks post-implantation. Tumour burden was assessed from manually segmented regions.RESULTS: All four imaging systems provided sufficient contrast to detect tumours in all of the mice after two weeks. No significant difference was detected between tumour doubling times estimated by low-field MRI, ultrasound imaging or high-field MRI. A strong correlation was measured between high-field MRI estimates of tumour burden and all the other modalities (p < 0.001, Pearson).CONCLUSION: These results suggest that both low-field MRI and ultrasound imaging are accurate modalities for characterising the growth of preclinical tumour models.

AB - BACKGROUND: Research using orthotopic and transgenic models of cancer requires imaging methods to non-invasively quantify tumour burden. As the choice of appropriate imaging modality is wide-ranging, this study aimed to compare low-field (1T) magnetic resonance imaging (MRI), a novel and relatively low-cost system, against established preclinical techniques: bioluminescence imaging (BLI), ultrasound imaging (US), and high-field (9.4T) MRI.METHODS: A model of colorectal metastasis to the liver was established in eight mice, which were imaged with each modality over four weeks post-implantation. Tumour burden was assessed from manually segmented regions.RESULTS: All four imaging systems provided sufficient contrast to detect tumours in all of the mice after two weeks. No significant difference was detected between tumour doubling times estimated by low-field MRI, ultrasound imaging or high-field MRI. A strong correlation was measured between high-field MRI estimates of tumour burden and all the other modalities (p < 0.001, Pearson).CONCLUSION: These results suggest that both low-field MRI and ultrasound imaging are accurate modalities for characterising the growth of preclinical tumour models.

KW - Animals

KW - Cell Line, Tumor

KW - Colorectal Neoplasms/diagnostic imaging

KW - Humans

KW - Luminescent Measurements/methods

KW - Lung Neoplasms/diagnostic imaging

KW - Magnetic Resonance Imaging/methods

KW - Mice

KW - Multimodal Imaging/methods

KW - Neoplasm Transplantation

KW - Transplantation, Heterologous

KW - Tumor Burden

KW - Ultrasonography/methods

U2 - 10.1371/journal.pone.0156162

DO - 10.1371/journal.pone.0156162

M3 - Article

C2 - 27223614

SN - 1932-6203

VL - 11

SP - e0156162

JO - PLoS ONE

JF - PLoS ONE

IS - 5

ER -