MosSCI-mediated exogenous gene expression is modulated by genomic positioning

Yuzhi Chen; Volker M. Arlt; Stephen R. Stürzenbaum

doi:10.1002/biot.202300062

MosSCI-mediated exogenous gene expression is modulated by genomic positioning

Yuzhi Chen, Volker M. Arlt, Stephen R. Stürzenbaum^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Although the Mos1-mediated single-copy insertion (MosSCI) technique has been widely used to generate stable transgenic Caenorhabditis elegans strains, the link between stability of expression and integration site still needs to be explored. Here, experimental evidence is provided that transgenes are not able to match the level of transcription of their native counterpart, and that insertions at certain locations can result in an external stress-mediated increase in expression. Insertion site ttTi5605 on chromosome II was shown to be a superior location, at least when introducing reproduction related genes. Thus, this study provides a reference for the selection of an optimal site for MosSCI which provides acceptable expression performance whilst minimizing undesirable secondary effects.

Original language	English
Article number	2300062
Journal	Biotechnology Journal
Volume	18
Issue number	9
DOIs	https://doi.org/10.1002/biot.202300062
Publication status	Published - Sept 2023

Keywords

Caenorhabditis elegans
genetic engineering
single-copy insertion
transcription

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10.1002/biot.202300062

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abstract = "Although the Mos1-mediated single-copy insertion (MosSCI) technique has been widely used to generate stable transgenic Caenorhabditis elegans strains, the link between stability of expression and integration site still needs to be explored. Here, experimental evidence is provided that transgenes are not able to match the level of transcription of their native counterpart, and that insertions at certain locations can result in an external stress-mediated increase in expression. Insertion site ttTi5605 on chromosome II was shown to be a superior location, at least when introducing reproduction related genes. Thus, this study provides a reference for the selection of an optimal site for MosSCI which provides acceptable expression performance whilst minimizing undesirable secondary effects.",

keywords = "Caenorhabditis elegans, genetic engineering, single-copy insertion, transcription",

author = "Yuzhi Chen and Arlt, {Volker M.} and St{\"u}rzenbaum, {Stephen R.}",

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AU - Arlt, Volker M.

AU - Stürzenbaum, Stephen R.

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AB - Although the Mos1-mediated single-copy insertion (MosSCI) technique has been widely used to generate stable transgenic Caenorhabditis elegans strains, the link between stability of expression and integration site still needs to be explored. Here, experimental evidence is provided that transgenes are not able to match the level of transcription of their native counterpart, and that insertions at certain locations can result in an external stress-mediated increase in expression. Insertion site ttTi5605 on chromosome II was shown to be a superior location, at least when introducing reproduction related genes. Thus, this study provides a reference for the selection of an optimal site for MosSCI which provides acceptable expression performance whilst minimizing undesirable secondary effects.

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MosSCI-mediated exogenous gene expression is modulated by genomic positioning

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