mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions

Jakob Jeriha; Nikola Kolundzic; Preeti Khurana; Andrea Perez-Dominguez; Dusko Ilic

doi:10.1007/7651_2020_302

mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions

Jakob Jeriha, Nikola Kolundzic, Preeti Khurana, Andrea Perez-Dominguez, Dusko Ilic

Women & Children's Health

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA).

Original language	English
Pages (from-to)	665-674
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2454
DOIs	https://doi.org/10.1007/7651_2020_302
Publication status	Published - 2022

Keywords

Chemically defined
Feeder-free
iPSC
miRNA
mRNA
Reprogramming
Xeno-free

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10.1007/7651_2020_302

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title = "mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions",

abstract = "Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA).",

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author = "Jakob Jeriha and Nikola Kolundzic and Preeti Khurana and Andrea Perez-Dominguez and Dusko Ilic",

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year = "2022",

doi = "10.1007/7651_2020_302",

language = "English",

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AU - Jeriha, Jakob

AU - Kolundzic, Nikola

AU - Khurana, Preeti

AU - Perez-Dominguez, Andrea

AU - Ilic, Dusko

N1 - Funding Information: N.K., P.K. and this work were supported by LEO Foundation grant number LF16028. J.J. is supported by the National Institute for Health Research (NIHR) Biomedical Research Centre based at Guy’s and St Thomas’ NHS Foundation trust and King’s College London. The views expressed are of the authors and not necessarily those of NHS, the NIHR or the Department of Health. Publisher Copyright: © 2020, Springer Science+Business Media New York.

PY - 2022

Y1 - 2022

N2 - Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA).

AB - Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA).

KW - Chemically defined

KW - Feeder-free

KW - iPSC

KW - miRNA

KW - mRNA

KW - Reprogramming

KW - Xeno-free

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions

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Keywords

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