Mucosal immunization with DNA vaccines

P Hobson; C Barnfield; A Barnes; L S Klavinskis

doi:10.1016/S1046-2023(03)00139-7

Mucosal immunization with DNA vaccines

P Hobson
, C Barnfield
, A Barnes
, L S Klavinskis

Peter Gorer Department of Immunobiology

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

DNA vaccines represent a new approach to the control of infectious disease. Both cellular and humoral immune responses are induced without the attendant concerns associated with live, attenuated vaccines. The vast majority of DNA vaccines are delivered by parental routes, which rarely elicit immune responses at the mucosal epithelia, the primary sites of pathogen transmission. In view of the importance of mucosal and regional lymph node immunity in the control of pathogens transmitted across the mucosal epithelia, a number of groups, including our own, have developed immunization strategies that target plasmid DNA to mucosal inductive sites associated with the lymphoid tissues of the respiratory, gastrointestinal, and genital tracts. Here, we describe the procedures for the formulation and delivery of plasmid DNA to mucosal inductive tissues and address the theoretical basis to selection of particular mucosal locations for the induction of effective immune responses. (C) 2003 Elsevier Science (USA). All rights reserved.

Original language	English
Pages (from-to)	217 - 224
Number of pages	8
Journal	Methods
Volume	31
Issue number	3
DOIs	https://doi.org/10.1016/S1046-2023(03)00139-7
Publication status	Published - 1 Nov 2003

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/S1046-2023(03)00139-7

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AU - Barnfield, C

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AU - Klavinskis, L S

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AB - DNA vaccines represent a new approach to the control of infectious disease. Both cellular and humoral immune responses are induced without the attendant concerns associated with live, attenuated vaccines. The vast majority of DNA vaccines are delivered by parental routes, which rarely elicit immune responses at the mucosal epithelia, the primary sites of pathogen transmission. In view of the importance of mucosal and regional lymph node immunity in the control of pathogens transmitted across the mucosal epithelia, a number of groups, including our own, have developed immunization strategies that target plasmid DNA to mucosal inductive sites associated with the lymphoid tissues of the respiratory, gastrointestinal, and genital tracts. Here, we describe the procedures for the formulation and delivery of plasmid DNA to mucosal inductive tissues and address the theoretical basis to selection of particular mucosal locations for the induction of effective immune responses. (C) 2003 Elsevier Science (USA). All rights reserved.

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Mucosal immunization with DNA vaccines

Abstract

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