Multi-scale spectrally resolved quantitative fluorescence imaging system: Towards neurosurgical guidance in glioma resection

TY - CHAP

T1 - Multi-scale spectrally resolved quantitative fluorescence imaging system

T2 - Clinical and Translational Neurophotonics

AU - Xie, Yijing

AU - Thom, Maria

AU - Miserocchi, Anna

AU - McEvoy, Andrew W.

AU - Desjardins, Adrien

AU - Ourselin, Sebastien

AU - Vercauteren, Tom

PY - 2017/2/27

Y1 - 2017/2/27

N2 - In glioma resection surgery, the detection of tumour is often guided by using intraoperative fluorescence imaging notably with 5-ALA-PpIX, providing fluorescent contrast between normal brain tissue and the gliomas tissue to achieve improved tumour delineation and prolonged patient survival compared with the conventional white-light guided resection. However, the commercially available fluorescence imaging system relies on surgeon's eyes to visualise and distinguish the fluorescence signals, which unfortunately makes the resection subjective. In this study, we developed a novel multi-scale spectrally-resolved fluorescence imaging system and a computational model for quantification of PpIX concentration. The system consisted of a wide-field spectrally-resolved quantitative imaging device and a fluorescence endomicroscopic imaging system enabling optical biopsy. Ex vivo animal tissue experiments as well as human tumour sample studies demonstrated that the system was capable of specifically detecting the PpIX fluorescent signal and estimate the true concentration of PpIX in brain specimen.

AB - In glioma resection surgery, the detection of tumour is often guided by using intraoperative fluorescence imaging notably with 5-ALA-PpIX, providing fluorescent contrast between normal brain tissue and the gliomas tissue to achieve improved tumour delineation and prolonged patient survival compared with the conventional white-light guided resection. However, the commercially available fluorescence imaging system relies on surgeon's eyes to visualise and distinguish the fluorescence signals, which unfortunately makes the resection subjective. In this study, we developed a novel multi-scale spectrally-resolved fluorescence imaging system and a computational model for quantification of PpIX concentration. The system consisted of a wide-field spectrally-resolved quantitative imaging device and a fluorescence endomicroscopic imaging system enabling optical biopsy. Ex vivo animal tissue experiments as well as human tumour sample studies demonstrated that the system was capable of specifically detecting the PpIX fluorescent signal and estimate the true concentration of PpIX in brain specimen.

KW - 5-ALA-PpIX

KW - Glioma resection

KW - Quantitative uorescence imaging

KW - Uorescence confocal laser endomicroscope

UR - https://www.scopus.com/pages/publications/85019237793

U2 - 10.1117/12.2250159

DO - 10.1117/12.2250159

M3 - Conference paper

AN - SCOPUS:85019237793

VL - 10050

BT - Clinical and Translational Neurophotonics

PB - SPIE

Y2 - 28 January 2017 through 29 January 2017

ER -