Multi-site optical excitation using ChR2 and micro-LED array

TY - JOUR

T1 - Multi-site optical excitation using ChR2 and micro-LED array

AU - Grossman, Nir

AU - Poher, Vincent

AU - Grubb, Matthew S.

AU - Kennedy, Gordon T.

AU - Nikolic, Konstantin

AU - McGovern, Brian

AU - Palmini, Rolando Berlinguer

AU - Gong, Zheng

AU - Drakakis, Emmanuel M.

AU - Neil, Mark A. A.

AU - Dawson, Martin D.

AU - Burrone, Juan

AU - Degenaar, Patrick

PY - 2010

Y1 - 2010

N2 - Studying neuronal processes such as synaptic summation, dendritic physiology and neural network dynamics requires complex spatiotemporal control over neuronal activities. The recent development of neural photosensitization tools, such as channelrhodopsin-2 (ChR2), offers new opportunities for non-invasive, flexible and cell-specific neuronal stimulation. Previously, complex spatiotemporal control of photosensitized neurons has been limited by the lack of appropriate optical devices which can provide 2D stimulation with sufficient irradiance. Here we present a simple and powerful solution that is based on an array of high-power micro light-emitting diodes (micro-LEDs) that can generate arbitrary optical excitation patterns on a neuronal sample with micrometre and millisecond resolution. We first describe the design and fabrication of the system and characterize its capabilities. We then demonstrate its capacity to elicit precise electrophysiological responses in cultured and slice neurons expressing ChR2.

AB - Studying neuronal processes such as synaptic summation, dendritic physiology and neural network dynamics requires complex spatiotemporal control over neuronal activities. The recent development of neural photosensitization tools, such as channelrhodopsin-2 (ChR2), offers new opportunities for non-invasive, flexible and cell-specific neuronal stimulation. Previously, complex spatiotemporal control of photosensitized neurons has been limited by the lack of appropriate optical devices which can provide 2D stimulation with sufficient irradiance. Here we present a simple and powerful solution that is based on an array of high-power micro light-emitting diodes (micro-LEDs) that can generate arbitrary optical excitation patterns on a neuronal sample with micrometre and millisecond resolution. We first describe the design and fabrication of the system and characterize its capabilities. We then demonstrate its capacity to elicit precise electrophysiological responses in cultured and slice neurons expressing ChR2.

U2 - 10.1088/1741-2560/7/1/016004

DO - 10.1088/1741-2560/7/1/016004

M3 - Article

VL - 7

JO - Journal Of Neural Engineering

JF - Journal Of Neural Engineering

IS - 1

M1 - 016004

ER -