Multifocal multiphoton volumetric imaging approach for high-speed time-resolved Förster resonance energy transfer imaging in vivo

Simon P. Poland; Grace K. Chan; James A. Levitt; Nikola Krstajić; Ahmet T. Erdogan; Robert K. Henderson; Maddy Parsons; Simon M. Ameer-Beg

doi:10.1364/OL.43.006057

Multifocal multiphoton volumetric imaging approach for high-speed time-resolved Förster resonance energy transfer imaging in vivo

Simon P. Poland^*, Grace K. Chan, James A. Levitt, Nikola Krstajić, Ahmet T. Erdogan, Robert K. Henderson, Maddy Parsons, Simon M. Ameer-Beg

^*Corresponding author for this work

Medical Schoo

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

In this Letter, we will discuss the development of a multifocal multiphoton fluorescent lifetime imaging system where four individual fluorescent intensity and lifetime planes are acquired simultaneously, allowing us to obtain volumetric data without the need for sequential scanning at different axial depths. Using a phase-only spatial light modulator (SLM) with an appropriate algorithm to generate a holographic pattern, we project a beamlet array within a sample volume of a size, which can be preprogrammed by the user. We demonstrate the capabilities of the system to image live-cell interactions. While only four planes are shown, this technique can be rescaled to a large number of focal planes, enabling full 3D acquisition and reconstruction.

Original language	English
Pages (from-to)	6057-6060
Number of pages	4
Journal	Optics Letters
Volume	43
Issue number	24
DOIs	https://doi.org/10.1364/OL.43.006057
Publication status	Published - 15 Dec 2018

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title = "Multifocal multiphoton volumetric imaging approach for high-speed time-resolved F{\"o}rster resonance energy transfer imaging in vivo",

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T1 - Multifocal multiphoton volumetric imaging approach for high-speed time-resolved Förster resonance energy transfer imaging in vivo

AU - Poland, Simon P.

AU - Chan, Grace K.

AU - Levitt, James A.

AU - Krstajić, Nikola

AU - Erdogan, Ahmet T.

AU - Henderson, Robert K.

AU - Parsons, Maddy

AU - Ameer-Beg, Simon M.

PY - 2018/12/15

Y1 - 2018/12/15

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AB - In this Letter, we will discuss the development of a multifocal multiphoton fluorescent lifetime imaging system where four individual fluorescent intensity and lifetime planes are acquired simultaneously, allowing us to obtain volumetric data without the need for sequential scanning at different axial depths. Using a phase-only spatial light modulator (SLM) with an appropriate algorithm to generate a holographic pattern, we project a beamlet array within a sample volume of a size, which can be preprogrammed by the user. We demonstrate the capabilities of the system to image live-cell interactions. While only four planes are shown, this technique can be rescaled to a large number of focal planes, enabling full 3D acquisition and reconstruction.

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JO - Optics Letters

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ER -

Multifocal multiphoton volumetric imaging approach for high-speed time-resolved Förster resonance energy transfer imaging in vivo

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