Multiple functions of tail-anchor domains of mitochondrial outer membrane proteins

Shukry Habib; Andreja Vasiljev; Walter Neupert; Doron Rapaport

doi:10.1016/S0014-5793(03)01325-5

Multiple functions of tail-anchor domains of mitochondrial outer membrane proteins

Shukry Habib, Andreja Vasiljev, Walter Neupert, Doron Rapaport

Gene Therapy and Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Tail-anchored proteins form a distinct class of membrane proteins that have a single membrane anchor sequence at their C-terminus, the tail-anchor. Their N-terminal portion is exposed to the cytosol. We have studied the roles of tail-anchor domains of proteins residing in the mitochondrial outer membrane. Four distinct functions of the tail-anchor domain were identified. First, the domain mediates the targeting to mitochondria in a process that probably requires a net positive charge at the C-terminally flanking segment. Second, tail-anchor domains facilitate the insertion into the mitochondrial outer membrane. Third, the tail-anchor is responsible for the assembly of the respective protein into functional multi-subunit complexes; and fourth, tail-anchor domains can stabilize such complexes.

Original language	English
Pages (from-to)	511-515
Number of pages	5
Journal	FEBS Letters
Volume	555
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(03)01325-5
Publication status	Published - 18 Dec 2003

Keywords

Amino Acid Sequence
Cytosol
Fungal Proteins
Genetic Complementation Test
Intracellular Membranes
Membrane Proteins
Mitochondrial Proteins
Molecular Sequence Data
Phenotype
Protein Sorting Signals
Protein Structure, Tertiary
Recombinant Fusion Proteins
Saccharomyces cerevisiae

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10.1016/S0014-5793(03)01325-5

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title = "Multiple functions of tail-anchor domains of mitochondrial outer membrane proteins",

abstract = "Tail-anchored proteins form a distinct class of membrane proteins that have a single membrane anchor sequence at their C-terminus, the tail-anchor. Their N-terminal portion is exposed to the cytosol. We have studied the roles of tail-anchor domains of proteins residing in the mitochondrial outer membrane. Four distinct functions of the tail-anchor domain were identified. First, the domain mediates the targeting to mitochondria in a process that probably requires a net positive charge at the C-terminally flanking segment. Second, tail-anchor domains facilitate the insertion into the mitochondrial outer membrane. Third, the tail-anchor is responsible for the assembly of the respective protein into functional multi-subunit complexes; and fourth, tail-anchor domains can stabilize such complexes.",

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T1 - Multiple functions of tail-anchor domains of mitochondrial outer membrane proteins

AU - Habib, Shukry

AU - Vasiljev, Andreja

AU - Neupert, Walter

AU - Rapaport, Doron

PY - 2003/12/18

Y1 - 2003/12/18

N2 - Tail-anchored proteins form a distinct class of membrane proteins that have a single membrane anchor sequence at their C-terminus, the tail-anchor. Their N-terminal portion is exposed to the cytosol. We have studied the roles of tail-anchor domains of proteins residing in the mitochondrial outer membrane. Four distinct functions of the tail-anchor domain were identified. First, the domain mediates the targeting to mitochondria in a process that probably requires a net positive charge at the C-terminally flanking segment. Second, tail-anchor domains facilitate the insertion into the mitochondrial outer membrane. Third, the tail-anchor is responsible for the assembly of the respective protein into functional multi-subunit complexes; and fourth, tail-anchor domains can stabilize such complexes.

AB - Tail-anchored proteins form a distinct class of membrane proteins that have a single membrane anchor sequence at their C-terminus, the tail-anchor. Their N-terminal portion is exposed to the cytosol. We have studied the roles of tail-anchor domains of proteins residing in the mitochondrial outer membrane. Four distinct functions of the tail-anchor domain were identified. First, the domain mediates the targeting to mitochondria in a process that probably requires a net positive charge at the C-terminally flanking segment. Second, tail-anchor domains facilitate the insertion into the mitochondrial outer membrane. Third, the tail-anchor is responsible for the assembly of the respective protein into functional multi-subunit complexes; and fourth, tail-anchor domains can stabilize such complexes.

KW - Amino Acid Sequence

KW - Cytosol

KW - Fungal Proteins

KW - Genetic Complementation Test

KW - Intracellular Membranes

KW - Membrane Proteins

KW - Mitochondrial Proteins

KW - Molecular Sequence Data

KW - Phenotype

KW - Protein Sorting Signals

KW - Protein Structure, Tertiary

KW - Recombinant Fusion Proteins

KW - Saccharomyces cerevisiae

U2 - 10.1016/S0014-5793(03)01325-5

DO - 10.1016/S0014-5793(03)01325-5

M3 - Article

C2 - 14675765

SN - 0014-5793

VL - 555

SP - 511

EP - 515

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Multiple functions of tail-anchor domains of mitochondrial outer membrane proteins

Abstract

Keywords

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