Multiplex ligation-dependent probe amplification using a completely synthetic probe set

R F Stern, R G Roberts, K Mann, S C Yau, J Berg, C Mackie Ogilvie

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

The recent development of multiplex ligation-dependent probe amplification (MLPA) has provided an efficient and reliable assay for dosage screening of multiple loci in a single reaction. However; a drawback to this method is the time-consuming process of generating a probe set by cloning in single-stranded bacteriophage vectors. We have developed a synthetic probe set to screen for deletions in a region spanning 18.5 Mb within chromosome 3q. In a pilot study, we tested 15 synthetic probes on 4 control samples and on 2 patients previously found to possess a heteroygous deletion in the region 3q26-q28. These synthetic probes detected deletions at all previously known deleted loci. Furthermore, using synthetic probes, the variability of results within samples was similar to that reported for commercially available M13-derived probes. Our results demonstrate that this novel approach to MLPA provides a generic solution to the difficulties of probe development by cloning; such synthetically generated probes may be used to screen a large number of loci in a single reaction. We conclude that the use of synthetic probes for MLPA is a rapid, robust, and efficient alternative for research (and potentially diagnostic) deletion and duplication screening of multiple genomic loci.
Original languageEnglish
Pages (from-to)399 - 405
Number of pages7
JournalBiotechniques
Volume37
Issue number3
Publication statusPublished - Sept 2004

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