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Multiplexed polarised hypodermic Raman needle probe for biostructural analysis of articular cartilage

Research output: Contribution to journalLetter

Original languageEnglish
JournalOptics Letters
Publication statusAccepted/In press - 15 Apr 2020

King's Authors

Abstract

We report a multiplexed polarised hypodermic Raman needle probe for the biostructural analysis of articular cartilage. Using a custom-developed needle probe with a sapphire ball lens, we measure polarised Raman spectra of cartilage. By imaging two polarisations simultaneously on the CCD and binning them separately, we capture both biochemical and structural tissue information in real time. Here, we demonstrate that polarised Raman spectroscopy can distinguish between different collagen fibril alignment orientations in a cartilage explant model system, supporting its capacity for diagnosing the hallmark collagen alignment changes that occur in the early stages of osteoarthritis (OA). Accordingly, this work shows that needle-based polarised Raman spectroscopy has great potential for the monitoring and diagnosis of early OA.

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