Muscle Stem Cell Fate Is Controlled by the Cell-Polarity Protein Scrib

Yusuke Ono*, Yoshishige Urata, Shinji Goto, Shunsuke Nakagawa, Patrick O. Humbert, Tao Sheng Li, Peter S. Zammit

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)
153 Downloads (Pure)

Abstract

Satellite cells are resident skeletal muscle stem cells that supply myonuclei for homeostasis, hypertrophy, and repair in adult muscle. Scrib is one of the major cell-polarity proteins, acting as a potent tumor suppressor in epithelial cells. Here, we show that Scrib also controls satellite-cell-fate decisions in adult mice. Scrib is undetectable in quiescent cells but becomes expressed during activation. Scrib isasymmetrically distributed in dividing daughter cells, with robust accumulation in cells committed to myogenic differentiation. Low Scrib expression is associated with the proliferative state and preventing self-renewal, whereas high Scrib levels reduce satellite cell proliferation. Satellite-cell-specific knockout of Scrib in mice causes a drastic andinsurmountable defect in muscle regeneration. Thus, Scrib is a regulator of tissue stem cells, controlling population expansion and self-renewal with Scrib expression dynamics directing satellite cell fate.

Original languageEnglish
Pages (from-to)1135-1148
Number of pages14
JournalCell Reports
Volume10
Issue number7
Early online date19 Feb 2015
DOIs
Publication statusPublished - 24 Feb 2015

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