Mutation and analysis of Dan, the founding member of the Dan family of transforming growth factor beta antagonists

TY - JOUR

T1 - Mutation and analysis of Dan, the founding member of the Dan family of transforming growth factor beta antagonists

AU - Dionne, M S

AU - Skarnes, W C

AU - Harland, R M

PY - 2001

Y1 - 2001

N2 - The Dan family of transforming growth factor beta antagonists is a large, evolutionarily conserved family of proteins. Little is known about either the specificity of these antagonists or the biological roles of these proteins. We have characterized Dan, the founding member of this family, with regard to both its biochemical specificity and its biological roles. Although DAN is not an efficient antagonist of BMP-2/4 class signals, we found that DAN was able to interact with GDF-5 in a frog embryo assay, suggesting that DAN may regulate signaling by the GDF-5/6/7 class of BMPs in vivo. Intriguingly, in developing neurons, Dan mRNA was localized to axons, suggesting a potential role for the DAN protein in axonal outgrowth or guidance. Mice lacking Dan activity were generated by gene targeting and displayed subtle, background-dependent defects

AB - The Dan family of transforming growth factor beta antagonists is a large, evolutionarily conserved family of proteins. Little is known about either the specificity of these antagonists or the biological roles of these proteins. We have characterized Dan, the founding member of this family, with regard to both its biochemical specificity and its biological roles. Although DAN is not an efficient antagonist of BMP-2/4 class signals, we found that DAN was able to interact with GDF-5 in a frog embryo assay, suggesting that DAN may regulate signaling by the GDF-5/6/7 class of BMPs in vivo. Intriguingly, in developing neurons, Dan mRNA was localized to axons, suggesting a potential role for the DAN protein in axonal outgrowth or guidance. Mice lacking Dan activity were generated by gene targeting and displayed subtle, background-dependent defects

U2 - 10.1128/MCB.21.2.636-643.2001

DO - 10.1128/MCB.21.2.636-643.2001

M3 - Article

VL - 21

SP - 636

EP - 643

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

IS - 2

ER -