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MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression

Research output: Contribution to journalArticlepeer-review

Juan F. Aranda, Natalia Reglero-Real, Beatriz Marcos-Ramiro, Ana Ruiz-Saenz, Laura Fernandez-Martin, Miguel Bernabe-Rubio, Leonor Kremer, Anne J. Ridley, Isabel Correas, Miguel A. Alonso, Jaime Millan

Original languageEnglish
Article numberN/A
Pages (from-to)483-494
Number of pages12
JournalMol Biol Cell
Volume24
Issue number4
DOIs
Published15 Feb 2013

King's Authors

Abstract

The endothelium maintains a barrier between blood and tissue that becomes more permeable during inflammation. Membrane rafts are ordered assemblies of cholesterol, glycolipids, and proteins that modulate proinflammatory cell signaling and barrier function. In epithelial cells, the MAL family members MAL, MAL2, and myeloid-associated differentiation marker (MYADM) regulate the function and dynamics of ordered membrane domains. We analyzed the expression of these three proteins in human endothelial cells and found that only MYADM is expressed. MYADM was confined in ordered domains at the plasma membrane, where it partially colocalized with filamentous actin and cell-cell junctions. Small interfering RNA (siRNA)-mediated MYADM knockdown increased permeability, ICAM-1 expression, and leukocyte adhesion, all of which are features of an inflammatory response. Barrier function decrease in MYADM-silenced cells was dependent on ICAM-1 expression. Membrane domains and the underlying actin cytoskeleton can regulate each other and are connected by ezrin, radixin, and moesin (ERM) proteins. In endothelial cells, MYADM knockdown induced ERM activation. Triple-ERM knockdown partially inhibited ICAM-1 increase induced by MYADM siRNA. Importantly, ERM knockdown also reduced ICAM-1 expression in response to the proinflammatory cytokine tumor necrosis factor-a. MYADM therefore regulates the connection between the plasma membrane and the cortical cytoskeleton and so can control the endothelial inflammatory response.

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