Myogenin promotes myocyte fusion to balance fibre number and size

TY - JOUR

T1 - Myogenin promotes myocyte fusion to balance fibre number and size

AU - Ganassi, Massimo

AU - Badodi, Sara

AU - Ortuste Quiroga, Huascar Pedro

AU - Zammit, Peter S.

AU - Hinits, Yaniv

AU - Hughes, Simon M.

PY - 2018/10/12

Y1 - 2018/10/12

N2 - Each skeletal muscle acquires its unique size before birth, when terminally differentiating myocytes fuse to form a defined number of multinucleated myofibres. Although mice in which the transcription factor Myogenin is mutated lack most myogenesis and die perinatally, a specific cell biological role for Myogenin has remained elusive. We report that loss of function of zebrafish myog prevents formation of almost all multinucleated muscle fibres. A second, Myog-independent, fusion pathway requires Hedgehog signalling. Lack of Myog does not prevent terminal differentiation; the smaller myotome has a normal number of myocytes forming more mononuclear, thin, albeit functional, fast muscle fibres. Mechanistically, Myogenin binds the myomaker promoter and is required for expression of myomaker and other genes essential for myocyte fusion. Adult myog mutants display reduced muscle mass, decreased fibre size and nucleation. Adult-derived myog mutant myocytes show persistent defective fusion ex vivo. Myogenin is therefore essential for muscle homeostasis, regulating myocyte fusion to determine both muscle fibre number and size.

AB - Each skeletal muscle acquires its unique size before birth, when terminally differentiating myocytes fuse to form a defined number of multinucleated myofibres. Although mice in which the transcription factor Myogenin is mutated lack most myogenesis and die perinatally, a specific cell biological role for Myogenin has remained elusive. We report that loss of function of zebrafish myog prevents formation of almost all multinucleated muscle fibres. A second, Myog-independent, fusion pathway requires Hedgehog signalling. Lack of Myog does not prevent terminal differentiation; the smaller myotome has a normal number of myocytes forming more mononuclear, thin, albeit functional, fast muscle fibres. Mechanistically, Myogenin binds the myomaker promoter and is required for expression of myomaker and other genes essential for myocyte fusion. Adult myog mutants display reduced muscle mass, decreased fibre size and nucleation. Adult-derived myog mutant myocytes show persistent defective fusion ex vivo. Myogenin is therefore essential for muscle homeostasis, regulating myocyte fusion to determine both muscle fibre number and size.

U2 - 10.1038/s41467-018-06583-6

DO - 10.1038/s41467-018-06583-6

M3 - Article

SN - 2041-1723

VL - 9

SP - 1

EP - 17

JO - Nature Communications

JF - Nature Communications

M1 - 4232

ER -