@article{0ea7901b457e4fc6a3c664757b672c56,
title = "Myosin-based regulation of twitch and tetanic contractions in mammalian skeletal muscle",
abstract = "Time-resolved X-ray diffraction from isolated fast-twitch muscles of the mouse was used to show how structural changes in the myosin-containing thick filaments contribute to the regulation of muscle contraction, extending the previous focus on regulation by the actin-containing thin filaments. This study shows that muscle activation involves the following sequence of structural changes: thin filament activation, disruption of the helical array of myosin motors characteristic of resting muscle, release of myosin motor domains from the folded conformation on the filament backbone, and actin attachment. Physiological force generation in the {\textquoteleft}twitch{\textquoteright} response of skeletal muscle to single action potential stimulation is limited by incomplete activation of the thick filament and the rapid inactivation of both filaments. Muscle relaxation after repetitive stimulation is accompanied by complete recovery of the folded motor conformation on the filament backbone but incomplete reformation of the helical array, revealing a structural basis for post-tetanic potentiation in isolated muscle.",
keywords = "Skeletal muscle, Muscle regulation, Muscle contraction, Myosin, X-ray diffraction",
author = "Cameron Hill and Elisabetta Brunello and Luca Fusi and {Garcia Ovejero}, Jesus and Malcolm Irving",
note = "Funding Information: We thank I22 staff, Olga Shebanova, Tim Snow and Nick Terrill (Diamond Light Source) and MRC Harwell for support during the beamtime; Diamond Light Source for the provision of synchrotron beamtime; Marty Rajaratnam (King?s College London) for mechanical engineering support and Thomas Kavanagh (King?s College London) for helping with 3Dprinting. This work was funded by the Medical Research Council MR/R01700X/1 and Diamond Light Source. EB and JGO were supported by a British Heart Foundation Intermediate Basic Science Research Fellowship awarded to EB (FS/17/3/32604). LF was funded by a Sir Henry Dale Fellowship awarded by the Wellcome Trust and the Royal Society (210464/Z/18/Z). Funding Information: This work was funded by the Medical Research Funding Information: Light Source 堀 EB and JGO were supported by a British Haert Foundation Publisher Copyright: {\textcopyright} 2021, eLife Sciences Publications Ltd. All rights reserved. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = jun,
doi = "10.7554/eLife.68211",
language = "English",
volume = "10",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications, Ltd",
}