Myosin VI Localization and Expression in Striated Muscle Pathology

Justyna Karolczak, Serge Weis, Elisabeth Ehler, Biruta Kierdaszuk, Mariusz Berdynski, Cezary Zekanowski, Anna M. Kaminska, Maria Jolanta Redowicz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Myosin VI (MVI) is a unique unconventional myosin translocating, unlike other myosins, towards the minus end of actin filaments. It is involved in numerous cellular processes such as endocytosis, intracellular trafficking, cell migration, and transcription. In mammalian skeletal muscles it localizes mainly to sarcoplasmic reticulum and is also present within the muscle nuclei and at the neuromuscular junction (Karolczak et al. Histochem Cell Biol 2013; 23:219-228). We have also shown that in denervated rat hindlimb muscle the MVI expression level is significantly increased and its localization is changed, indicating an important role of MVI in striated muscle pathology. Here, we addressed this problem by examining the distribution and expression levels of myosin VI in biopsies of skeletal muscles from patients with different myopathies. We found that, particularly in myopathies associated with fiber atrophy, the amount of MVI was enhanced and its localization in affected fibers was changed. Also, since a mutation within the human MVI gene was shown to be associated with cardiomyopathy, we assessed MVI localization and expression level in cardiac muscle using wild type and MLP(2/2) mice, a dilated cardiomyopathy model. No significant difference in MVI expression level was observed for both types of animals. MVI was found at intercalated discs and also at the sarcoplasmic reticulum. In the knockout mice, it was also present in ring-like structures surrounding the nuclei. The data indicate that in striated muscle MVI could be engaged in sarcoplasmic reticulum maintenance and/or functioning, vesicular transport, signal transmission and possibly in gene transcription.

Original languageEnglish
Pages (from-to)1706-1713
Number of pages8
JournalAnatomical record-Advances in integrative anatomy and evolutionary biology
Issue number9
Publication statusPublished - Sept 2014


  • myosin VI
  • unconventional myosin
  • muscle pathology
  • skeletal muscle
  • cardiac muscle
  • sarcoplasmic reticulum
  • PC12 CELLS
  • GENE
  • MICE


Dive into the research topics of 'Myosin VI Localization and Expression in Striated Muscle Pathology'. Together they form a unique fingerprint.

Cite this