N-acetyl aspartate estimation: a potential method for determining neuronal loss in the transmissible spongiform encephalopathies

Y L Chung; J Barr; K Bhakoo; S C R Williams; J D Bell; J R Fraser

doi:10.1046/j.1365-2990.2003.00438.x

N-acetyl aspartate estimation: a potential method for determining neuronal loss in the transmissible spongiform encephalopathies

Y L Chung, J Barr, K Bhakoo, S C R Williams, J D Bell, J R Fraser

Neuroimaging

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Neurodegenerative pathology is typical of the transmissible spongiform encephalopathies (TSEs), and is thought to underlie clinical disease. Some morphometric studies have shown early focal neurone loss, but the full extent of TSE induced neuronal loss in the central nervous system is not known, and can only be accurately estimated using intensive morphometric techniques. We have used a murine scrapie model in which we determined the levels of N-acetyl aspartate (NAA), a putative neuronal marker, by both high-performance liquid chromatography and high resolution, proton magnetic resonance spectroscopy in samples taken sequentially from the hippocampus. This scrapie model develops severe neuronal loss in the hippocampus, and the NAA levels showed a significant positive correlation with our previous morphometric estimates of neurone number. NAA measurement may therefore provide a practical alternative to intensive morphometric techniques in the investigation of neurodegeneration in the TSEs.

Original language	English
Pages (from-to)	445 - 450
Number of pages	6
Journal	Neuropathology and Applied Neurobiology
Volume	29
Issue number	5
DOIs	https://doi.org/10.1046/j.1365-2990.2003.00438.x
Publication status	Published - Oct 2003

Access to Document

10.1046/j.1365-2990.2003.00438.x

Cite this

@article{9c023d093e6d4de397e2e0763ff7ee77,

title = "N-acetyl aspartate estimation: a potential method for determining neuronal loss in the transmissible spongiform encephalopathies",

abstract = "Neurodegenerative pathology is typical of the transmissible spongiform encephalopathies (TSEs), and is thought to underlie clinical disease. Some morphometric studies have shown early focal neurone loss, but the full extent of TSE induced neuronal loss in the central nervous system is not known, and can only be accurately estimated using intensive morphometric techniques. We have used a murine scrapie model in which we determined the levels of N-acetyl aspartate (NAA), a putative neuronal marker, by both high-performance liquid chromatography and high resolution, proton magnetic resonance spectroscopy in samples taken sequentially from the hippocampus. This scrapie model develops severe neuronal loss in the hippocampus, and the NAA levels showed a significant positive correlation with our previous morphometric estimates of neurone number. NAA measurement may therefore provide a practical alternative to intensive morphometric techniques in the investigation of neurodegeneration in the TSEs.",

author = "Chung, {Y L} and J Barr and K Bhakoo and Williams, {S C R} and Bell, {J D} and Fraser, {J R}",

year = "2003",

month = oct,

doi = "10.1046/j.1365-2990.2003.00438.x",

language = "English",

volume = "29",

pages = "445 -- 450",

journal = "Neuropathology and Applied Neurobiology",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - N-acetyl aspartate estimation: a potential method for determining neuronal loss in the transmissible spongiform encephalopathies

AU - Chung, Y L

AU - Barr, J

AU - Bhakoo, K

AU - Williams, S C R

AU - Bell, J D

AU - Fraser, J R

PY - 2003/10

Y1 - 2003/10

N2 - Neurodegenerative pathology is typical of the transmissible spongiform encephalopathies (TSEs), and is thought to underlie clinical disease. Some morphometric studies have shown early focal neurone loss, but the full extent of TSE induced neuronal loss in the central nervous system is not known, and can only be accurately estimated using intensive morphometric techniques. We have used a murine scrapie model in which we determined the levels of N-acetyl aspartate (NAA), a putative neuronal marker, by both high-performance liquid chromatography and high resolution, proton magnetic resonance spectroscopy in samples taken sequentially from the hippocampus. This scrapie model develops severe neuronal loss in the hippocampus, and the NAA levels showed a significant positive correlation with our previous morphometric estimates of neurone number. NAA measurement may therefore provide a practical alternative to intensive morphometric techniques in the investigation of neurodegeneration in the TSEs.

AB - Neurodegenerative pathology is typical of the transmissible spongiform encephalopathies (TSEs), and is thought to underlie clinical disease. Some morphometric studies have shown early focal neurone loss, but the full extent of TSE induced neuronal loss in the central nervous system is not known, and can only be accurately estimated using intensive morphometric techniques. We have used a murine scrapie model in which we determined the levels of N-acetyl aspartate (NAA), a putative neuronal marker, by both high-performance liquid chromatography and high resolution, proton magnetic resonance spectroscopy in samples taken sequentially from the hippocampus. This scrapie model develops severe neuronal loss in the hippocampus, and the NAA levels showed a significant positive correlation with our previous morphometric estimates of neurone number. NAA measurement may therefore provide a practical alternative to intensive morphometric techniques in the investigation of neurodegeneration in the TSEs.

UR - http://www.scopus.com/inward/record.url?scp=0141596006&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2990.2003.00438.x

DO - 10.1046/j.1365-2990.2003.00438.x

M3 - Article

VL - 29

SP - 445

EP - 450

JO - Neuropathology and Applied Neurobiology

JF - Neuropathology and Applied Neurobiology

IS - 5

ER -

N-acetyl aspartate estimation: a potential method for determining neuronal loss in the transmissible spongiform encephalopathies

Abstract

Access to Document

Other files and links

Fingerprint

Cite this