Nanoneedles Induce Targeted siRNA Silencing of p16 in the Human Corneal Endothelium

Eleonora Maurizi; Davide Alessandro Martella; Davide Schiroli; Alessia Merra; Salman Ahmad Mustfa; Graziella Pellegrini; Claudio Macaluso; Ciro Chiappini

doi:10.1002/advs.202203257

Nanoneedles Induce Targeted siRNA Silencing of p16 in the Human Corneal Endothelium

Eleonora Maurizi, Davide Alessandro Martella, Davide Schiroli, Alessia Merra, Salman Ahmad Mustfa, Graziella Pellegrini, Claudio Macaluso, Ciro Chiappini

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Abstract

Nanoneedles can target nucleic acid transfection to primary cells at tissue interfaces with high efficiency and minimal perturbation. The corneal endothelium is an ideal target for nanoneedle-mediated RNA interference therapy aimed at enhancing its proliferative capacity, necessary for tissue regeneration. This work develops a strategy for siRNA nanoninjection to the human corneal endothelium. Nanoneedles can deliver p16-targeting siRNA to primary human corneal endothelial cells in vitro without toxicity. The nanoinjection of siRNA induces p16 silencing and increases cell proliferation, as monitored by ki67 expression. Furthermore, siRNA nanoinjection targeting the human corneal endothelium is nontoxic ex vivo, and silences p16 in transfected cells. These data indicate that nanoinjection can support targeted RNA interference therapy for the treatment of endothelial corneal dysfunction.

Original language	English
Pages (from-to)	e2203257
Journal	Advanced Science
Volume	9
Issue number	33
Early online date	17 Oct 2022
DOIs	https://doi.org/10.1002/advs.202203257
Publication status	Published - 24 Nov 2022

Keywords

Humans
RNA, Small Interfering/genetics
Endothelium, Corneal/metabolism
Endothelial Cells/metabolism
Transfection
Cell Proliferation

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10.1002/advs.202203257Licence: CC BY

Nanoneedles Induce Targeted_MAURIZI_Publishedonline17October2022_GOLD VoR (CC BY)Final published version, 5.12 MBLicence: CC BY

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title = "Nanoneedles Induce Targeted siRNA Silencing of p16 in the Human Corneal Endothelium",

abstract = "Nanoneedles can target nucleic acid transfection to primary cells at tissue interfaces with high efficiency and minimal perturbation. The corneal endothelium is an ideal target for nanoneedle-mediated RNA interference therapy aimed at enhancing its proliferative capacity, necessary for tissue regeneration. This work develops a strategy for siRNA nanoninjection to the human corneal endothelium. Nanoneedles can deliver p16-targeting siRNA to primary human corneal endothelial cells in vitro without toxicity. The nanoinjection of siRNA induces p16 silencing and increases cell proliferation, as monitored by ki67 expression. Furthermore, siRNA nanoinjection targeting the human corneal endothelium is nontoxic ex vivo, and silences p16 in transfected cells. These data indicate that nanoinjection can support targeted RNA interference therapy for the treatment of endothelial corneal dysfunction.",

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author = "Eleonora Maurizi and Martella, {Davide Alessandro} and Davide Schiroli and Alessia Merra and Mustfa, {Salman Ahmad} and Graziella Pellegrini and Claudio Macaluso and Ciro Chiappini",

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doi = "10.1002/advs.202203257",

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T1 - Nanoneedles Induce Targeted siRNA Silencing of p16 in the Human Corneal Endothelium

AU - Maurizi, Eleonora

AU - Martella, Davide Alessandro

AU - Schiroli, Davide

AU - Merra, Alessia

AU - Mustfa, Salman Ahmad

AU - Pellegrini, Graziella

AU - Macaluso, Claudio

AU - Chiappini, Ciro

PY - 2022/11/24

Y1 - 2022/11/24

N2 - Nanoneedles can target nucleic acid transfection to primary cells at tissue interfaces with high efficiency and minimal perturbation. The corneal endothelium is an ideal target for nanoneedle-mediated RNA interference therapy aimed at enhancing its proliferative capacity, necessary for tissue regeneration. This work develops a strategy for siRNA nanoninjection to the human corneal endothelium. Nanoneedles can deliver p16-targeting siRNA to primary human corneal endothelial cells in vitro without toxicity. The nanoinjection of siRNA induces p16 silencing and increases cell proliferation, as monitored by ki67 expression. Furthermore, siRNA nanoinjection targeting the human corneal endothelium is nontoxic ex vivo, and silences p16 in transfected cells. These data indicate that nanoinjection can support targeted RNA interference therapy for the treatment of endothelial corneal dysfunction.

AB - Nanoneedles can target nucleic acid transfection to primary cells at tissue interfaces with high efficiency and minimal perturbation. The corneal endothelium is an ideal target for nanoneedle-mediated RNA interference therapy aimed at enhancing its proliferative capacity, necessary for tissue regeneration. This work develops a strategy for siRNA nanoninjection to the human corneal endothelium. Nanoneedles can deliver p16-targeting siRNA to primary human corneal endothelial cells in vitro without toxicity. The nanoinjection of siRNA induces p16 silencing and increases cell proliferation, as monitored by ki67 expression. Furthermore, siRNA nanoinjection targeting the human corneal endothelium is nontoxic ex vivo, and silences p16 in transfected cells. These data indicate that nanoinjection can support targeted RNA interference therapy for the treatment of endothelial corneal dysfunction.

KW - Humans

KW - RNA, Small Interfering/genetics

KW - Endothelium, Corneal/metabolism

KW - Endothelial Cells/metabolism

KW - Transfection

KW - Cell Proliferation

U2 - 10.1002/advs.202203257

DO - 10.1002/advs.202203257

M3 - Article

C2 - 36253148

SN - 2198-3844

VL - 9

SP - e2203257

JO - Advanced Science

JF - Advanced Science

IS - 33

ER -

Nanoneedles Induce Targeted siRNA Silencing of p16 in the Human Corneal Endothelium

Abstract

Keywords

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