Nanoparticles from lipid-based liquid crystals: Emulsifier influence on morphology and cytotoxicity

Sergio Murgia; Angela M. Falchi; Miguel Mano; Sandrina Lampis; Rossella Angius; Anna M. Carnerup; Judith Schmidt; Giacomo Diaz; Mauro Giacca; Yeshayahu Talmon; Maura Monduzzi

doi:10.1021/jp9098655

Nanoparticles from lipid-based liquid crystals: Emulsifier influence on morphology and cytotoxicity

Sergio Murgia, Angela M. Falchi, Miguel Mano, Sandrina Lampis, Rossella Angius, Anna M. Carnerup, Judith Schmidt, Giacomo Diaz, Mauro Giacca, Yeshayahu Talmon, Maura Monduzzi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

100 Citations (Scopus)

Abstract

Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) and lauroylcholine chloride (LCh), are investigated for structural features and for short-term in vitro cytotoxicity. Depending on the emulsifiers, different morphologies of the lipid NPs (cubosomes and liposomes) are obtained, as demonstrated by cryoTEM images. Although NPs offer many advantages in medical applications and various chemicals used for their preparation are under investigation, so far there are no standardized procedures to evaluate cell biocompatibility. Two different protocols to evaluate the impact of these lipid NPs on biological systems are presented. Results show that nanoparticles stabilized by PF127 (cubosomes) display a relevant toxicity toward different cell lines, whereas those stabilized by LCh (liposomes) affect cell viability at a much lesser extent.

Original language	English
Pages (from-to)	3518-3525
Number of pages	8
Journal	Journal of Physical Chemistry B
Volume	114
Issue number	10
DOIs	https://doi.org/10.1021/jp9098655
Publication status	Published - 18 Mar 2010

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title = "Nanoparticles from lipid-based liquid crystals: Emulsifier influence on morphology and cytotoxicity",

abstract = "Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) and lauroylcholine chloride (LCh), are investigated for structural features and for short-term in vitro cytotoxicity. Depending on the emulsifiers, different morphologies of the lipid NPs (cubosomes and liposomes) are obtained, as demonstrated by cryoTEM images. Although NPs offer many advantages in medical applications and various chemicals used for their preparation are under investigation, so far there are no standardized procedures to evaluate cell biocompatibility. Two different protocols to evaluate the impact of these lipid NPs on biological systems are presented. Results show that nanoparticles stabilized by PF127 (cubosomes) display a relevant toxicity toward different cell lines, whereas those stabilized by LCh (liposomes) affect cell viability at a much lesser extent.",

author = "Sergio Murgia and Falchi, {Angela M.} and Miguel Mano and Sandrina Lampis and Rossella Angius and Carnerup, {Anna M.} and Judith Schmidt and Giacomo Diaz and Mauro Giacca and Yeshayahu Talmon and Maura Monduzzi",

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T1 - Nanoparticles from lipid-based liquid crystals

T2 - Emulsifier influence on morphology and cytotoxicity

AU - Murgia, Sergio

AU - Falchi, Angela M.

AU - Mano, Miguel

AU - Lampis, Sandrina

AU - Angius, Rossella

AU - Carnerup, Anna M.

AU - Schmidt, Judith

AU - Diaz, Giacomo

AU - Giacca, Mauro

AU - Talmon, Yeshayahu

AU - Monduzzi, Maura

PY - 2010/3/18

Y1 - 2010/3/18

N2 - Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) and lauroylcholine chloride (LCh), are investigated for structural features and for short-term in vitro cytotoxicity. Depending on the emulsifiers, different morphologies of the lipid NPs (cubosomes and liposomes) are obtained, as demonstrated by cryoTEM images. Although NPs offer many advantages in medical applications and various chemicals used for their preparation are under investigation, so far there are no standardized procedures to evaluate cell biocompatibility. Two different protocols to evaluate the impact of these lipid NPs on biological systems are presented. Results show that nanoparticles stabilized by PF127 (cubosomes) display a relevant toxicity toward different cell lines, whereas those stabilized by LCh (liposomes) affect cell viability at a much lesser extent.

AB - Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) and lauroylcholine chloride (LCh), are investigated for structural features and for short-term in vitro cytotoxicity. Depending on the emulsifiers, different morphologies of the lipid NPs (cubosomes and liposomes) are obtained, as demonstrated by cryoTEM images. Although NPs offer many advantages in medical applications and various chemicals used for their preparation are under investigation, so far there are no standardized procedures to evaluate cell biocompatibility. Two different protocols to evaluate the impact of these lipid NPs on biological systems are presented. Results show that nanoparticles stabilized by PF127 (cubosomes) display a relevant toxicity toward different cell lines, whereas those stabilized by LCh (liposomes) affect cell viability at a much lesser extent.

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IS - 10

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Nanoparticles from lipid-based liquid crystals: Emulsifier influence on morphology and cytotoxicity

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