Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs

Nils Hellwig; Oliver Peetz; Zainab Ahdash; Igor Tascón; Paula J. Booth; Vedrana Mikusevic; Marina Diskowski; Argyris Politis; Yvonne Hellmich; Inga Hänelt; Eamonn Reading; Nina Morgner

doi:10.1039/c8cc06284f

Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs

Nils Hellwig, Oliver Peetz, Zainab Ahdash, Igor Tascón, Paula J. Booth, Vedrana Mikusevic, Marina Diskowski, Argyris Politis, Yvonne Hellmich, Inga Hänelt, Eamonn Reading, Nina Morgner^*

^*Corresponding author for this work

Goethe University Frankfurt

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

238 Downloads (Pure)

Abstract

Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.

Original language	English
Pages (from-to)	13702-13705
Number of pages	4
Journal	Chemical communications (Cambridge, England)
Volume	54
Issue number	97
Early online date	19 Nov 2018
DOIs	https://doi.org/10.1039/c8cc06284f
Publication status	Published - 2018

Keywords

Membrane Proteins
Mass Spectrometry
POLYMERS
LIPIDS

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10.1039/c8cc06284fLicence: CC BY

Native mass spectrometry_HELLWIG_Accepted6Sep2018_GOLD VoRFinal published version, 3.05 MBLicence: CC BY

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Hellwig, N., Peetz, O., Ahdash, Z., Tascón, I., Booth, P. J., Mikusevic, V., Diskowski, M., Politis, A., Hellmich, Y., Hänelt, I., Reading, E., & Morgner, N. (2018). Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs. Chemical communications (Cambridge, England), 54(97), 13702-13705. https://doi.org/10.1039/c8cc06284f

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title = "Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs",

abstract = "Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.",

keywords = "Membrane Proteins, Mass Spectrometry, POLYMERS, LIPIDS",

author = "Nils Hellwig and Oliver Peetz and Zainab Ahdash and Igor Tasc{\'o}n and Booth, {Paula J.} and Vedrana Mikusevic and Marina Diskowski and Argyris Politis and Yvonne Hellmich and Inga H{\"a}nelt and Eamonn Reading and Nina Morgner",

year = "2018",

doi = "10.1039/c8cc06284f",

language = "English",

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pages = "13702--13705",

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Hellwig, N, Peetz, O, Ahdash, Z, Tascón, I, Booth, PJ, Mikusevic, V, Diskowski, M, Politis, A, Hellmich, Y, Hänelt, I, Reading, E & Morgner, N 2018, 'Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs', Chemical communications (Cambridge, England), vol. 54, no. 97, pp. 13702-13705. https://doi.org/10.1039/c8cc06284f

TY - JOUR

T1 - Native mass spectrometry goes more native

T2 - Investigation of membrane protein complexes directly from SMALPs

AU - Hellwig, Nils

AU - Peetz, Oliver

AU - Ahdash, Zainab

AU - Tascón, Igor

AU - Booth, Paula J.

AU - Mikusevic, Vedrana

AU - Diskowski, Marina

AU - Politis, Argyris

AU - Hellmich, Yvonne

AU - Hänelt, Inga

AU - Reading, Eamonn

AU - Morgner, Nina

PY - 2018

Y1 - 2018

N2 - Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.

AB - Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.

KW - Membrane Proteins

KW - Mass Spectrometry

KW - POLYMERS

KW - LIPIDS

UR - http://www.scopus.com/inward/record.url?scp=85058146864&partnerID=8YFLogxK

U2 - 10.1039/c8cc06284f

DO - 10.1039/c8cc06284f

M3 - Article

C2 - 30452022

SN - 1359-7345

VL - 54

SP - 13702

EP - 13705

JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

IS - 97

ER -

Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs

Abstract

Keywords

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