Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs

Nils Hellwig, Oliver Peetz, Zainab Ahdash, Igor Tascón, Paula J. Booth, Vedrana Mikusevic, Marina Diskowski, Argyris Politis, Yvonne Hellmich, Inga Hänelt, Eamonn Reading, Nina Morgner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)
208 Downloads (Pure)

Abstract

Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.

Original languageEnglish
Pages (from-to)13702-13705
Number of pages4
JournalChemical communications (Cambridge, England)
Volume54
Issue number97
Early online date19 Nov 2018
DOIs
Publication statusPublished - 2018

Keywords

  • Membrane Proteins
  • Mass Spectrometry
  • POLYMERS
  • LIPIDS

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