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Negative evidence for a functional role of neuronal DNMT3a in persistent pain

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Negative evidence for a functional role of neuronal DNMT3a in persistent pain. / Saunders, Jessica; Hore, Zoe; Gentry, Clive et al.

In: Frontiers in Molecular Neuroscience, Vol. 11, 332, 12.09.2018.

Research output: Contribution to journalArticlepeer-review

Harvard

Saunders, J, Hore, Z, Gentry, C, McMahon, S & Denk, F 2018, 'Negative evidence for a functional role of neuronal DNMT3a in persistent pain', Frontiers in Molecular Neuroscience, vol. 11, 332. https://doi.org/10.3389/fnmol.2018.00332

APA

Saunders, J., Hore, Z., Gentry, C., McMahon, S., & Denk, F. (2018). Negative evidence for a functional role of neuronal DNMT3a in persistent pain. Frontiers in Molecular Neuroscience, 11, [332]. https://doi.org/10.3389/fnmol.2018.00332

Vancouver

Saunders J, Hore Z, Gentry C, McMahon S, Denk F. Negative evidence for a functional role of neuronal DNMT3a in persistent pain. Frontiers in Molecular Neuroscience. 2018 Sep 12;11. 332. https://doi.org/10.3389/fnmol.2018.00332

Author

Saunders, Jessica ; Hore, Zoe ; Gentry, Clive et al. / Negative evidence for a functional role of neuronal DNMT3a in persistent pain. In: Frontiers in Molecular Neuroscience. 2018 ; Vol. 11.

Bibtex Download

@article{72a9a012ec0a4b4390d7969d986b7364,
title = "Negative evidence for a functional role of neuronal DNMT3a in persistent pain",
abstract = "Traditionally, neuroscience has had to rely on mixed tissue analysis to examinetranscriptional and epigenetic changes in the context of nervous system function orpathology. However, particularly when studying chronic pain conditions, this approachcan be flawed, since it neglects to take into account the shifting contribution of differentcell types across experimental conditions. Here, we demonstrate this using the exampleof DNA methyltransferases (DNMTs) – a group of epigenetic modifiers consisting ofDnmt1, Dnmt3a, and Dnmt3b in mammalian cells. We used sensory neuron-specificknockout mice for Dnmt3a/3b as well as pharmacological blockade of Dnmt1 to studytheir role in nociception. In contrast to previous analyses on whole tissue, we find thatDnmt3a and 3b protein is not expressed in adult DRG neurons, that none of the DNAmethyltransferases are regulated with injury and that interfering with their function has noeffect on nociception. Our results therefore currently do not support a role for neuronalDNA methyltransferases in pain processing in adult animals.",
author = "Jessica Saunders and Zoe Hore and Clive Gentry and Stephen McMahon and Franziska Denk",
year = "2018",
month = sep,
day = "12",
doi = "10.3389/fnmol.2018.00332",
language = "English",
volume = "11",
journal = "Frontiers in Molecular Neuroscience",
issn = "1662-5099",
publisher = "Frontiers Media",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Negative evidence for a functional role of neuronal DNMT3a in persistent pain

AU - Saunders, Jessica

AU - Hore, Zoe

AU - Gentry, Clive

AU - McMahon, Stephen

AU - Denk, Franziska

PY - 2018/9/12

Y1 - 2018/9/12

N2 - Traditionally, neuroscience has had to rely on mixed tissue analysis to examinetranscriptional and epigenetic changes in the context of nervous system function orpathology. However, particularly when studying chronic pain conditions, this approachcan be flawed, since it neglects to take into account the shifting contribution of differentcell types across experimental conditions. Here, we demonstrate this using the exampleof DNA methyltransferases (DNMTs) – a group of epigenetic modifiers consisting ofDnmt1, Dnmt3a, and Dnmt3b in mammalian cells. We used sensory neuron-specificknockout mice for Dnmt3a/3b as well as pharmacological blockade of Dnmt1 to studytheir role in nociception. In contrast to previous analyses on whole tissue, we find thatDnmt3a and 3b protein is not expressed in adult DRG neurons, that none of the DNAmethyltransferases are regulated with injury and that interfering with their function has noeffect on nociception. Our results therefore currently do not support a role for neuronalDNA methyltransferases in pain processing in adult animals.

AB - Traditionally, neuroscience has had to rely on mixed tissue analysis to examinetranscriptional and epigenetic changes in the context of nervous system function orpathology. However, particularly when studying chronic pain conditions, this approachcan be flawed, since it neglects to take into account the shifting contribution of differentcell types across experimental conditions. Here, we demonstrate this using the exampleof DNA methyltransferases (DNMTs) – a group of epigenetic modifiers consisting ofDnmt1, Dnmt3a, and Dnmt3b in mammalian cells. We used sensory neuron-specificknockout mice for Dnmt3a/3b as well as pharmacological blockade of Dnmt1 to studytheir role in nociception. In contrast to previous analyses on whole tissue, we find thatDnmt3a and 3b protein is not expressed in adult DRG neurons, that none of the DNAmethyltransferases are regulated with injury and that interfering with their function has noeffect on nociception. Our results therefore currently do not support a role for neuronalDNA methyltransferases in pain processing in adult animals.

UR - http://www.scopus.com/inward/record.url?scp=85054825632&partnerID=8YFLogxK

U2 - 10.3389/fnmol.2018.00332

DO - 10.3389/fnmol.2018.00332

M3 - Article

VL - 11

JO - Frontiers in Molecular Neuroscience

JF - Frontiers in Molecular Neuroscience

SN - 1662-5099

M1 - 332

ER -

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