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Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle

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Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle. / Holt, Ian; Fuller, Heidi R.; Lam, Le Thanh; Sewry, Caroline A.; Shirran, Sally L.; Zhang, Qiuping; Shanahan, Catherine M.; Morris, Glenn E.

In: Scientific Reports, Vol. 9, No. 1, 14202, 01.12.2019.

Research output: Contribution to journalArticle

Harvard

Holt, I, Fuller, HR, Lam, LT, Sewry, CA, Shirran, SL, Zhang, Q, Shanahan, CM & Morris, GE 2019, 'Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle', Scientific Reports, vol. 9, no. 1, 14202. https://doi.org/10.1038/s41598-019-50728-6

APA

Holt, I., Fuller, H. R., Lam, L. T., Sewry, C. A., Shirran, S. L., Zhang, Q., ... Morris, G. E. (2019). Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle. Scientific Reports, 9(1), [14202]. https://doi.org/10.1038/s41598-019-50728-6

Vancouver

Holt I, Fuller HR, Lam LT, Sewry CA, Shirran SL, Zhang Q et al. Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle. Scientific Reports. 2019 Dec 1;9(1). 14202. https://doi.org/10.1038/s41598-019-50728-6

Author

Holt, Ian ; Fuller, Heidi R. ; Lam, Le Thanh ; Sewry, Caroline A. ; Shirran, Sally L. ; Zhang, Qiuping ; Shanahan, Catherine M. ; Morris, Glenn E. / Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle. In: Scientific Reports. 2019 ; Vol. 9, No. 1.

Bibtex Download

@article{39f3e59f2fef4d3f8e39c1a2679ef042,
title = "Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle",
abstract = "Nesprins, nuclear envelope spectrin-repeat proteins encoded by the SYNE1 and SYNE2 genes, are involved in localization of nuclei. The short isoform, nesprin-1-alpha2, is required for relocation of the microtubule organizer function from centromeres to the nuclear rim during myogenesis. Using specific antibodies, we now show that both nesprin-1-alpha2 and nesprin-1-giant co-localize with kinesin at the junctions of concatenated nuclei and at the outer poles of nuclear chains in human skeletal myotubes. In adult muscle, nesprin-1-alpha2 was found, together with kinesin, only on nuclei associated with neuromuscular junctions, whereas all adult cardiomyocyte nuclei expressed nesprin-1-alpha2. In a proteomics study, kinesin heavy and light chains were the only significant proteins in myotube extracts pulled down by nesprin-1-alpha2, but not by a mutant lacking the highly-conserved STAR domain (18 amino-acids, including the LEWD motif). The results support a function for nesprin-1-alpha2 in the specific localization of skeletal muscle nuclei mediated by kinesins and suggest that its primary role is at the outer nuclear membrane.",
author = "Ian Holt and Fuller, {Heidi R.} and Lam, {Le Thanh} and Sewry, {Caroline A.} and Shirran, {Sally L.} and Qiuping Zhang and Shanahan, {Catherine M.} and Morris, {Glenn E.}",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-019-50728-6",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle

AU - Holt, Ian

AU - Fuller, Heidi R.

AU - Lam, Le Thanh

AU - Sewry, Caroline A.

AU - Shirran, Sally L.

AU - Zhang, Qiuping

AU - Shanahan, Catherine M.

AU - Morris, Glenn E.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Nesprins, nuclear envelope spectrin-repeat proteins encoded by the SYNE1 and SYNE2 genes, are involved in localization of nuclei. The short isoform, nesprin-1-alpha2, is required for relocation of the microtubule organizer function from centromeres to the nuclear rim during myogenesis. Using specific antibodies, we now show that both nesprin-1-alpha2 and nesprin-1-giant co-localize with kinesin at the junctions of concatenated nuclei and at the outer poles of nuclear chains in human skeletal myotubes. In adult muscle, nesprin-1-alpha2 was found, together with kinesin, only on nuclei associated with neuromuscular junctions, whereas all adult cardiomyocyte nuclei expressed nesprin-1-alpha2. In a proteomics study, kinesin heavy and light chains were the only significant proteins in myotube extracts pulled down by nesprin-1-alpha2, but not by a mutant lacking the highly-conserved STAR domain (18 amino-acids, including the LEWD motif). The results support a function for nesprin-1-alpha2 in the specific localization of skeletal muscle nuclei mediated by kinesins and suggest that its primary role is at the outer nuclear membrane.

AB - Nesprins, nuclear envelope spectrin-repeat proteins encoded by the SYNE1 and SYNE2 genes, are involved in localization of nuclei. The short isoform, nesprin-1-alpha2, is required for relocation of the microtubule organizer function from centromeres to the nuclear rim during myogenesis. Using specific antibodies, we now show that both nesprin-1-alpha2 and nesprin-1-giant co-localize with kinesin at the junctions of concatenated nuclei and at the outer poles of nuclear chains in human skeletal myotubes. In adult muscle, nesprin-1-alpha2 was found, together with kinesin, only on nuclei associated with neuromuscular junctions, whereas all adult cardiomyocyte nuclei expressed nesprin-1-alpha2. In a proteomics study, kinesin heavy and light chains were the only significant proteins in myotube extracts pulled down by nesprin-1-alpha2, but not by a mutant lacking the highly-conserved STAR domain (18 amino-acids, including the LEWD motif). The results support a function for nesprin-1-alpha2 in the specific localization of skeletal muscle nuclei mediated by kinesins and suggest that its primary role is at the outer nuclear membrane.

UR - http://www.scopus.com/inward/record.url?scp=85072909439&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-50728-6

DO - 10.1038/s41598-019-50728-6

M3 - Article

C2 - 31578382

AN - SCOPUS:85072909439

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 14202

ER -

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