Neuron-Specific Mitochondrial DNA Deletion Levels in Sporadic Alzheimer's Disease

Anne Gerschuetz; Helmut Heinsen; Edna Gruenblatt; Anne K. Wagner; Jasmin Bartl; Christoph Meissner; Andreas J. Fallgatter; Safa Al-Sarraj; Claire Troakes; Isidro Ferrer; Thomas Arzberger; Juergen Deckert; Peter Riederer; Matthias Fischer; Thomas Tatschner; Camelia M. Monoranu

Neuron-Specific Mitochondrial DNA Deletion Levels in Sporadic Alzheimer's Disease

Anne Gerschuetz, Helmut Heinsen, Edna Gruenblatt, Anne K. Wagner, Jasmin Bartl, Christoph Meissner, Andreas J. Fallgatter, Safa Al-Sarraj, Claire Troakes, Isidro Ferrer, Thomas Arzberger, Juergen Deckert, Peter Riederer, Matthias Fischer, Thomas Tatschner, Camelia M. Monoranu^*

^*Corresponding author for this work

Basic and Clinical Neuroscience

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Oxidative stress is implicated in the pathogenesis of neurodegenerative diseases, including sporadic Alzheimer's disease (AD). Mitochondrial DNA (mtDNA) deletions are markers of oxidative damage and increase with age. To unravel the impact of mtDNA damage on AD development, we analyzed mtDNA deletion levels in diverse neuronal cell types of four brain regions (hippocampal CA1 and CA2 regions, nucleus tractus spinalis nervi trigemini, and the cerebellum) that exhibit differing levels of vulnerability to AD related changes at progressive Braak stages compared with age-matched controls. Neurons from these four brain regions were collected using laser microdissection, and analyzed using quantitative polymerase chain reaction (qPCR). Although, no correlation between mtDNA deletion levels and AD progression were found, the data revealed regional and cell type specific selective vulnerability towards mtDNA deletion levels. In conclusion, unexpected results were obtained as granule cells from the cerebellum and neurons from the nucleus tractus spinalis nervi trigemini of the brain stem displayed significant higher mtDNA deletion levels than pyramidal cells from hippocampal CA1 and CA2 region in age and AD.

Original language	English
Pages (from-to)	1041-1046
Number of pages	6
Journal	Current Alzheimer Research
Volume	10
Issue number	10
Publication status	Published - Dec 2013

Keywords

Alzheimer's disease
deletion
neurodegeneration
neuronal mitochondrial DNA
oxidative stress
selective vulnerability
VAGUS NERVE-STIMULATION
FAR-FIELD POTENTIALS
BRAIN-STEM
OXIDATIVE STRESS
NEURODEGENERATIVE DISEASES
OXIDASE
PCR
DEGENERATION
HIPPOCAMPUS
DYSFUNCTION

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Gerschuetz, A., Heinsen, H., Gruenblatt, E., Wagner, A. K., Bartl, J., Meissner, C., Fallgatter, A. J., Al-Sarraj, S., Troakes, C., Ferrer, I., Arzberger, T., Deckert, J., Riederer, P., Fischer, M., Tatschner, T., & Monoranu, C. M. (2013). Neuron-Specific Mitochondrial DNA Deletion Levels in Sporadic Alzheimer's Disease. Current Alzheimer Research, 10(10), 1041-1046. http://www.ingentaconnect.com/content/ben/car/2013/00000010/00000010/art00003?token=0057173e232ab87275c277b42572b67567d6b253e7b2e7b2f592f653b2a2d3a7c4e724770b017917b815f5b

@article{c348f2ecca0149d5b06067246dd4473a,

title = "Neuron-Specific Mitochondrial DNA Deletion Levels in Sporadic Alzheimer's Disease",

abstract = "Oxidative stress is implicated in the pathogenesis of neurodegenerative diseases, including sporadic Alzheimer's disease (AD). Mitochondrial DNA (mtDNA) deletions are markers of oxidative damage and increase with age. To unravel the impact of mtDNA damage on AD development, we analyzed mtDNA deletion levels in diverse neuronal cell types of four brain regions (hippocampal CA1 and CA2 regions, nucleus tractus spinalis nervi trigemini, and the cerebellum) that exhibit differing levels of vulnerability to AD related changes at progressive Braak stages compared with age-matched controls. Neurons from these four brain regions were collected using laser microdissection, and analyzed using quantitative polymerase chain reaction (qPCR). Although, no correlation between mtDNA deletion levels and AD progression were found, the data revealed regional and cell type specific selective vulnerability towards mtDNA deletion levels. In conclusion, unexpected results were obtained as granule cells from the cerebellum and neurons from the nucleus tractus spinalis nervi trigemini of the brain stem displayed significant higher mtDNA deletion levels than pyramidal cells from hippocampal CA1 and CA2 region in age and AD.",

keywords = "Alzheimer's disease, deletion, neurodegeneration, neuronal mitochondrial DNA, oxidative stress, selective vulnerability, VAGUS NERVE-STIMULATION, FAR-FIELD POTENTIALS, BRAIN-STEM, OXIDATIVE STRESS, NEURODEGENERATIVE DISEASES, OXIDASE, PCR, DEGENERATION, HIPPOCAMPUS, DYSFUNCTION",

author = "Anne Gerschuetz and Helmut Heinsen and Edna Gruenblatt and Wagner, {Anne K.} and Jasmin Bartl and Christoph Meissner and Fallgatter, {Andreas J.} and Safa Al-Sarraj and Claire Troakes and Isidro Ferrer and Thomas Arzberger and Juergen Deckert and Peter Riederer and Matthias Fischer and Thomas Tatschner and Monoranu, {Camelia M.}",

year = "2013",

month = dec,

language = "English",

volume = "10",

pages = "1041--1046",

journal = "Current Alzheimer Research",

issn = "1567-2050",

publisher = "Bentham Science Publishers B.V.",

number = "10",

}

Gerschuetz, A, Heinsen, H, Gruenblatt, E, Wagner, AK, Bartl, J, Meissner, C, Fallgatter, AJ, Al-Sarraj, S , Troakes, C, Ferrer, I, Arzberger, T, Deckert, J, Riederer, P, Fischer, M, Tatschner, T & Monoranu, CM 2013, 'Neuron-Specific Mitochondrial DNA Deletion Levels in Sporadic Alzheimer's Disease', Current Alzheimer Research, vol. 10, no. 10, pp. 1041-1046. <http://www.ingentaconnect.com/content/ben/car/2013/00000010/00000010/art00003?token=0057173e232ab87275c277b42572b67567d6b253e7b2e7b2f592f653b2a2d3a7c4e724770b017917b815f5b>

TY - JOUR

T1 - Neuron-Specific Mitochondrial DNA Deletion Levels in Sporadic Alzheimer's Disease

AU - Gerschuetz, Anne

AU - Heinsen, Helmut

AU - Gruenblatt, Edna

AU - Wagner, Anne K.

AU - Bartl, Jasmin

AU - Meissner, Christoph

AU - Fallgatter, Andreas J.

AU - Al-Sarraj, Safa

AU - Troakes, Claire

AU - Ferrer, Isidro

AU - Arzberger, Thomas

AU - Deckert, Juergen

AU - Riederer, Peter

AU - Fischer, Matthias

AU - Tatschner, Thomas

AU - Monoranu, Camelia M.

PY - 2013/12

Y1 - 2013/12

N2 - Oxidative stress is implicated in the pathogenesis of neurodegenerative diseases, including sporadic Alzheimer's disease (AD). Mitochondrial DNA (mtDNA) deletions are markers of oxidative damage and increase with age. To unravel the impact of mtDNA damage on AD development, we analyzed mtDNA deletion levels in diverse neuronal cell types of four brain regions (hippocampal CA1 and CA2 regions, nucleus tractus spinalis nervi trigemini, and the cerebellum) that exhibit differing levels of vulnerability to AD related changes at progressive Braak stages compared with age-matched controls. Neurons from these four brain regions were collected using laser microdissection, and analyzed using quantitative polymerase chain reaction (qPCR). Although, no correlation between mtDNA deletion levels and AD progression were found, the data revealed regional and cell type specific selective vulnerability towards mtDNA deletion levels. In conclusion, unexpected results were obtained as granule cells from the cerebellum and neurons from the nucleus tractus spinalis nervi trigemini of the brain stem displayed significant higher mtDNA deletion levels than pyramidal cells from hippocampal CA1 and CA2 region in age and AD.

AB - Oxidative stress is implicated in the pathogenesis of neurodegenerative diseases, including sporadic Alzheimer's disease (AD). Mitochondrial DNA (mtDNA) deletions are markers of oxidative damage and increase with age. To unravel the impact of mtDNA damage on AD development, we analyzed mtDNA deletion levels in diverse neuronal cell types of four brain regions (hippocampal CA1 and CA2 regions, nucleus tractus spinalis nervi trigemini, and the cerebellum) that exhibit differing levels of vulnerability to AD related changes at progressive Braak stages compared with age-matched controls. Neurons from these four brain regions were collected using laser microdissection, and analyzed using quantitative polymerase chain reaction (qPCR). Although, no correlation between mtDNA deletion levels and AD progression were found, the data revealed regional and cell type specific selective vulnerability towards mtDNA deletion levels. In conclusion, unexpected results were obtained as granule cells from the cerebellum and neurons from the nucleus tractus spinalis nervi trigemini of the brain stem displayed significant higher mtDNA deletion levels than pyramidal cells from hippocampal CA1 and CA2 region in age and AD.

KW - Alzheimer's disease

KW - deletion

KW - neurodegeneration

KW - neuronal mitochondrial DNA

KW - oxidative stress

KW - selective vulnerability

KW - VAGUS NERVE-STIMULATION

KW - FAR-FIELD POTENTIALS

KW - BRAIN-STEM

KW - OXIDATIVE STRESS

KW - NEURODEGENERATIVE DISEASES

KW - OXIDASE

KW - PCR

KW - DEGENERATION

KW - HIPPOCAMPUS

KW - DYSFUNCTION

M3 - Article

SN - 1567-2050

VL - 10

SP - 1041

EP - 1046

JO - Current Alzheimer Research

JF - Current Alzheimer Research

IS - 10

ER -

Neuron-Specific Mitochondrial DNA Deletion Levels in Sporadic Alzheimer's Disease

Abstract

Keywords

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