Neuronal Receptors Display Cytoskeleton-Independent Directed Motion on the Plasma Membrane

TY - JOUR

T1 - Neuronal Receptors Display Cytoskeleton-Independent Directed Motion on the Plasma Membrane

AU - Taylor, Ruth D.

AU - Heine, Martin

AU - Emptage, Nigel J.

AU - Andreae, Laura C.

N1 - Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2018/12/21

Y1 - 2018/12/21

N2 - Directed transport of transmembrane proteins is generally believed to occur via intracellular transport vesicles. However, using single-particle tracking in rat hippocampal neurons with a pH-sensitive quantum dot probe that specifically reports surface movement of receptors, we have identified a subpopulation of neuronal EphB2 receptors that exhibit directed motion between synapses within the plasma membrane itself. This receptor movement occurs independently of the cytoskeleton but is dependent on cholesterol and is regulated by neuronal activity.

AB - Directed transport of transmembrane proteins is generally believed to occur via intracellular transport vesicles. However, using single-particle tracking in rat hippocampal neurons with a pH-sensitive quantum dot probe that specifically reports surface movement of receptors, we have identified a subpopulation of neuronal EphB2 receptors that exhibit directed motion between synapses within the plasma membrane itself. This receptor movement occurs independently of the cytoskeleton but is dependent on cholesterol and is regulated by neuronal activity.

U2 - 10.1016/j.isci.2018.12.001

DO - 10.1016/j.isci.2018.12.001

M3 - Article

C2 - 30557785

SN - 2589-0042

VL - 10

SP - 234

EP - 244

JO - iScience

JF - iScience

ER -