Neuropilin 1 and 2 control cranial gangliogenesis and axon guidance through neural crest cells

TY - JOUR

T1 - Neuropilin 1 and 2 control cranial gangliogenesis and axon guidance through neural crest cells

AU - Schwarz, Quenten

AU - Vieira, Joaquim M.

AU - Howard, Beatrice

AU - Eickholt, Britta J.

AU - Ruhrberg, Christiana

PY - 2008/5

Y1 - 2008/5

N2 - Neuropilin (NRP) receptors and their class 3 semaphorin (SEMA3) ligands play well-established roles in axon guidance, with loss of NRP1, NRP2, SEMA3A or SEMA3F causing defasciculation and errors in growth cone guidance of peripherally projecting nerves. Here we report that loss of NRP1 or NRP2 also impairs sensory neuron positioning in the mouse head, and that this defect is a consequence of inappropriate cranial neural crest cell migration. Specifically, neural crest cells move into the normally crest-free territory between the trigeminal and hyoid neural crest streams and recruit sensory neurons from the otic placode; these ectopic neurons then extend axons between the trigeminal and facioacoustic ganglia. Moreover, we found that NRP1 and NRP2 cooperate to guide cranial neural crest cells and position sensory neurons; thus, in the absence of SEMA3/NRP signalling, the segmentation of the cranial nervous system is lost. We conclude that neuropilins play multiple roles in the sensory nervous system by directing cranial neural crest cells, positioning sensory neurons and organising their axonal projections.

AB - Neuropilin (NRP) receptors and their class 3 semaphorin (SEMA3) ligands play well-established roles in axon guidance, with loss of NRP1, NRP2, SEMA3A or SEMA3F causing defasciculation and errors in growth cone guidance of peripherally projecting nerves. Here we report that loss of NRP1 or NRP2 also impairs sensory neuron positioning in the mouse head, and that this defect is a consequence of inappropriate cranial neural crest cell migration. Specifically, neural crest cells move into the normally crest-free territory between the trigeminal and hyoid neural crest streams and recruit sensory neurons from the otic placode; these ectopic neurons then extend axons between the trigeminal and facioacoustic ganglia. Moreover, we found that NRP1 and NRP2 cooperate to guide cranial neural crest cells and position sensory neurons; thus, in the absence of SEMA3/NRP signalling, the segmentation of the cranial nervous system is lost. We conclude that neuropilins play multiple roles in the sensory nervous system by directing cranial neural crest cells, positioning sensory neurons and organising their axonal projections.

KW - Axon guidance

KW - Mouse

KW - Neural crest cell

KW - Neuropilin

KW - Peripheral nervous system

KW - Placode

KW - Semaphorin

KW - Sensory neuron

UR - https://www.scopus.com/pages/publications/46049109310

U2 - 10.1242/dev.015412

DO - 10.1242/dev.015412

M3 - Article

C2 - 18356247

SN - 1477-9129

VL - 135

SP - 1605

EP - 1613

JO - Development (Cambridge): for advances in developmental biology and stem cells

JF - Development (Cambridge): for advances in developmental biology and stem cells

IS - 9

ER -