Nitric oxide generation mediated by beta-adrenoceptors is impaired in platelets from patients with Type 2 diabetes mellitus

TY - JOUR

T1 - Nitric oxide generation mediated by beta-adrenoceptors is impaired in platelets from patients with Type 2 diabetes mellitus

AU - Queen, L R

AU - Ji, Y

AU - Goubareva, I

AU - Ferro, A

PY - 2003/11

Y1 - 2003/11

N2 - Aims/hypothesis. Type 2 diabetic patients have been shown to have reduced basal platelet nitric oxide synthase activity, which is a possible contributor to the vascular complications seen in the disease. We investigated platelet nitric oxide generation stimulated by beta-adrenoceptors and adenylyl cyclase in Type 2 diabetic patients and control subjects. Methods. Platelets isolated from blood taken from nine Type 2 diabetic patients and nine healthy control subjects of similar age were treated with isoproterenol 1 mumol/l, forskolin 1 mumol/l or vehicle. Platelet nitric oxide synthase activity was measured by L-[H-3]-arginine to L-[H-3]-citrulline conversion, cyclic GMP content by radioimmunoassay, and nitric oxide synthase type 3 expression by western blotting. Results. Basal platelet nitric oxide synthase activity was lower in diabetic patients than in control subjects (0.01+/-0.02 pmol L-citrulline/10(8) platelets, compared with 0.12+/-0.05; p

AB - Aims/hypothesis. Type 2 diabetic patients have been shown to have reduced basal platelet nitric oxide synthase activity, which is a possible contributor to the vascular complications seen in the disease. We investigated platelet nitric oxide generation stimulated by beta-adrenoceptors and adenylyl cyclase in Type 2 diabetic patients and control subjects. Methods. Platelets isolated from blood taken from nine Type 2 diabetic patients and nine healthy control subjects of similar age were treated with isoproterenol 1 mumol/l, forskolin 1 mumol/l or vehicle. Platelet nitric oxide synthase activity was measured by L-[H-3]-arginine to L-[H-3]-citrulline conversion, cyclic GMP content by radioimmunoassay, and nitric oxide synthase type 3 expression by western blotting. Results. Basal platelet nitric oxide synthase activity was lower in diabetic patients than in control subjects (0.01+/-0.02 pmol L-citrulline/10(8) platelets, compared with 0.12+/-0.05; p

UR - http://www.scopus.com/inward/record.url?scp=0344255514&partnerID=8YFLogxK

U2 - 10.1007/s00125-003-1219-0

DO - 10.1007/s00125-003-1219-0

M3 - Article

VL - 46

SP - 1474

EP - 1482

JO - Diabetologia

JF - Diabetologia

IS - 11

ER -