Nitrosative stress induced cytotoxicity in Giardia intestinalis

D Lloyd; J C Harris; S Maroulis; A Mitchell; M N Hughes; R B Wadley; M R Edwards

doi:10.1046/j.1365-2672.2003.02008.x

Nitrosative stress induced cytotoxicity in Giardia intestinalis

D Lloyd, J C Harris, S Maroulis, A Mitchell, M N Hughes, R B Wadley, M R Edwards

Child & Family Health Nursing

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Aims: To investigate the antigiardial properties of the nitrosating agents: sodium nitrite, sodium nitroprusside and Roussin's black salt. Methods and Results: Use of confocal laser scanning microscopy and flow cytometry indicated permeabilization of the plasma membrane to the anionic fluorophore, DiBAC(4) (3) [bis(1,3-dibutylbarbituric acid) trimethine oxonol]. Loss of plasma membrane electrochemical potential was accompanied by loss of regulated cellular volume control. Changes in ultrastructure revealed by electron microscopy and capacity for oxygen consumption, were also consequences of nitrosative stress. Roussin's black salt (RBS), active at micromolar concentrations was the most potent of the three agents tested. Conclusions: These multitargeted cytotoxic agents affected plasma membrane functions, inhibited cellular functions in Giardia intestinalis and led to loss of viability. Significance and Impact of the Study: Nitrosative damage, as an antigiardial strategy, may have implications for development of chemotherapy along with suggesting natural host defence mechanisms.

Original language	English
Pages (from-to)	576 - 583
Number of pages	8
Journal	JOURNAL OF APPLIED MICROBIOLOGY
Volume	95
Issue number	3
DOIs	https://doi.org/10.1046/j.1365-2672.2003.02008.x
Publication status	Published - 2003

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title = "Nitrosative stress induced cytotoxicity in Giardia intestinalis",

abstract = "Aims: To investigate the antigiardial properties of the nitrosating agents: sodium nitrite, sodium nitroprusside and Roussin's black salt. Methods and Results: Use of confocal laser scanning microscopy and flow cytometry indicated permeabilization of the plasma membrane to the anionic fluorophore, DiBAC(4) (3) [bis(1,3-dibutylbarbituric acid) trimethine oxonol]. Loss of plasma membrane electrochemical potential was accompanied by loss of regulated cellular volume control. Changes in ultrastructure revealed by electron microscopy and capacity for oxygen consumption, were also consequences of nitrosative stress. Roussin's black salt (RBS), active at micromolar concentrations was the most potent of the three agents tested. Conclusions: These multitargeted cytotoxic agents affected plasma membrane functions, inhibited cellular functions in Giardia intestinalis and led to loss of viability. Significance and Impact of the Study: Nitrosative damage, as an antigiardial strategy, may have implications for development of chemotherapy along with suggesting natural host defence mechanisms.",

author = "D Lloyd and Harris, {J C} and S Maroulis and A Mitchell and Hughes, {M N} and Wadley, {R B} and Edwards, {M R}",

year = "2003",

doi = "10.1046/j.1365-2672.2003.02008.x",

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T1 - Nitrosative stress induced cytotoxicity in Giardia intestinalis

AU - Lloyd, D

AU - Harris, J C

AU - Maroulis, S

AU - Mitchell, A

AU - Hughes, M N

AU - Wadley, R B

AU - Edwards, M R

PY - 2003

Y1 - 2003

N2 - Aims: To investigate the antigiardial properties of the nitrosating agents: sodium nitrite, sodium nitroprusside and Roussin's black salt. Methods and Results: Use of confocal laser scanning microscopy and flow cytometry indicated permeabilization of the plasma membrane to the anionic fluorophore, DiBAC(4) (3) [bis(1,3-dibutylbarbituric acid) trimethine oxonol]. Loss of plasma membrane electrochemical potential was accompanied by loss of regulated cellular volume control. Changes in ultrastructure revealed by electron microscopy and capacity for oxygen consumption, were also consequences of nitrosative stress. Roussin's black salt (RBS), active at micromolar concentrations was the most potent of the three agents tested. Conclusions: These multitargeted cytotoxic agents affected plasma membrane functions, inhibited cellular functions in Giardia intestinalis and led to loss of viability. Significance and Impact of the Study: Nitrosative damage, as an antigiardial strategy, may have implications for development of chemotherapy along with suggesting natural host defence mechanisms.

AB - Aims: To investigate the antigiardial properties of the nitrosating agents: sodium nitrite, sodium nitroprusside and Roussin's black salt. Methods and Results: Use of confocal laser scanning microscopy and flow cytometry indicated permeabilization of the plasma membrane to the anionic fluorophore, DiBAC(4) (3) [bis(1,3-dibutylbarbituric acid) trimethine oxonol]. Loss of plasma membrane electrochemical potential was accompanied by loss of regulated cellular volume control. Changes in ultrastructure revealed by electron microscopy and capacity for oxygen consumption, were also consequences of nitrosative stress. Roussin's black salt (RBS), active at micromolar concentrations was the most potent of the three agents tested. Conclusions: These multitargeted cytotoxic agents affected plasma membrane functions, inhibited cellular functions in Giardia intestinalis and led to loss of viability. Significance and Impact of the Study: Nitrosative damage, as an antigiardial strategy, may have implications for development of chemotherapy along with suggesting natural host defence mechanisms.

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DO - 10.1046/j.1365-2672.2003.02008.x

M3 - Article

VL - 95

SP - 576

EP - 583

JO - JOURNAL OF APPLIED MICROBIOLOGY

JF - JOURNAL OF APPLIED MICROBIOLOGY

IS - 3

ER -

Nitrosative stress induced cytotoxicity in Giardia intestinalis

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