nlz gene family is required for hindbrain patterning in the zebrafish

Jacqueline Hoyle; Yixin P Tang; Elisabeth L Wiellette; Fiona C Wardle; Hazel L Sive

doi:10.1002/dvdy.20001

nlz gene family is required for hindbrain patterning in the zebrafish

Jacqueline Hoyle, Yixin P Tang, Elisabeth L Wiellette, Fiona C Wardle, Hazel L Sive

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

This study describes the conserved niz gene family whose members encode unusual zinc finger proteins. In the zebrafish neurectoderm, both nIz1 and the newly isolated nIz2 are expressed in the presumptive hindbrain and midbrain/hindbrain boundary, where expression of nIz1 is dependent on pax2a. In addition, nIz2 is uniquely expressed more anteriorly, in the presumptive midbrain and diencephalon. Overexpression of NIz proteins during gastrula stages inhibits hindbrain development. In particular, ectopically expressed NIz1 inhibits formation of future rhombomeres 2 and 3 (r2, r3), whereas neighboring r1 and r4 are not affected. Conversely, simultaneous reduction of NIz1 and NIz2 protein function by expression of antisense morpholino-modified oligomers leads to expansion of future r3 and r5, with associated loss of r4. These data indicate that one function of the niz gene family is to specify or maintain r4 identity, and to limit r3 and r5 during hindbrain formation. (C) 2004 Wiley-Liss, Inc.

Original language	English
Pages (from-to)	835 - 846
Number of pages	12
Journal	Developmental Dynamics
Volume	229
Issue number	4
DOIs	https://doi.org/10.1002/dvdy.20001
Publication status	Published - Apr 2004

Access to Document

10.1002/dvdy.20001

http://onlinelibrary.wiley.com/doi/10.1002/dvdy.20001/abstract;jsessionid=C12EAFD53311FDF104C6367AB0059FEF.d01t01?systemMessage=Due+to+scheduled+maintenance%2C+access+to+Wiley+Online+Library+will+be+disrupted+on+Saturday%2C+5th+Feb+between+10%3A00-12%3A00+GMT

Cite this

@article{5cb07bfb4f2c42bc81863305a08910fb,

title = "nlz gene family is required for hindbrain patterning in the zebrafish",

abstract = "This study describes the conserved niz gene family whose members encode unusual zinc finger proteins. In the zebrafish neurectoderm, both nIz1 and the newly isolated nIz2 are expressed in the presumptive hindbrain and midbrain/hindbrain boundary, where expression of nIz1 is dependent on pax2a. In addition, nIz2 is uniquely expressed more anteriorly, in the presumptive midbrain and diencephalon. Overexpression of NIz proteins during gastrula stages inhibits hindbrain development. In particular, ectopically expressed NIz1 inhibits formation of future rhombomeres 2 and 3 (r2, r3), whereas neighboring r1 and r4 are not affected. Conversely, simultaneous reduction of NIz1 and NIz2 protein function by expression of antisense morpholino-modified oligomers leads to expansion of future r3 and r5, with associated loss of r4. These data indicate that one function of the niz gene family is to specify or maintain r4 identity, and to limit r3 and r5 during hindbrain formation. (C) 2004 Wiley-Liss, Inc.",

author = "Jacqueline Hoyle and Tang, {Yixin P} and Wiellette, {Elisabeth L} and Wardle, {Fiona C} and Sive, {Hazel L}",

year = "2004",

month = apr,

doi = "10.1002/dvdy.20001",

language = "English",

volume = "229",

pages = "835 -- 846",

journal = "Developmental Dynamics",

issn = "1097-0177",

publisher = "Wiley-Liss Inc.",

number = "4",

}

TY - JOUR

T1 - nlz gene family is required for hindbrain patterning in the zebrafish

AU - Hoyle, Jacqueline

AU - Tang, Yixin P

AU - Wiellette, Elisabeth L

AU - Wardle, Fiona C

AU - Sive, Hazel L

PY - 2004/4

Y1 - 2004/4

N2 - This study describes the conserved niz gene family whose members encode unusual zinc finger proteins. In the zebrafish neurectoderm, both nIz1 and the newly isolated nIz2 are expressed in the presumptive hindbrain and midbrain/hindbrain boundary, where expression of nIz1 is dependent on pax2a. In addition, nIz2 is uniquely expressed more anteriorly, in the presumptive midbrain and diencephalon. Overexpression of NIz proteins during gastrula stages inhibits hindbrain development. In particular, ectopically expressed NIz1 inhibits formation of future rhombomeres 2 and 3 (r2, r3), whereas neighboring r1 and r4 are not affected. Conversely, simultaneous reduction of NIz1 and NIz2 protein function by expression of antisense morpholino-modified oligomers leads to expansion of future r3 and r5, with associated loss of r4. These data indicate that one function of the niz gene family is to specify or maintain r4 identity, and to limit r3 and r5 during hindbrain formation. (C) 2004 Wiley-Liss, Inc.

AB - This study describes the conserved niz gene family whose members encode unusual zinc finger proteins. In the zebrafish neurectoderm, both nIz1 and the newly isolated nIz2 are expressed in the presumptive hindbrain and midbrain/hindbrain boundary, where expression of nIz1 is dependent on pax2a. In addition, nIz2 is uniquely expressed more anteriorly, in the presumptive midbrain and diencephalon. Overexpression of NIz proteins during gastrula stages inhibits hindbrain development. In particular, ectopically expressed NIz1 inhibits formation of future rhombomeres 2 and 3 (r2, r3), whereas neighboring r1 and r4 are not affected. Conversely, simultaneous reduction of NIz1 and NIz2 protein function by expression of antisense morpholino-modified oligomers leads to expansion of future r3 and r5, with associated loss of r4. These data indicate that one function of the niz gene family is to specify or maintain r4 identity, and to limit r3 and r5 during hindbrain formation. (C) 2004 Wiley-Liss, Inc.

U2 - 10.1002/dvdy.20001

DO - 10.1002/dvdy.20001

M3 - Article

SN - 1097-0177

VL - 229

SP - 835

EP - 846

JO - Developmental Dynamics

JF - Developmental Dynamics

IS - 4

ER -

nlz gene family is required for hindbrain patterning in the zebrafish

Abstract

Access to Document

Fingerprint

Cite this