NMR Spectroscopy of Native and in Vitro Tissues Implicates PolyADP Ribose in Biomineralization

TY - JOUR

T1 - NMR Spectroscopy of Native and in Vitro Tissues Implicates PolyADP Ribose in Biomineralization

AU - Chow, W. Ying

AU - Rajan, Rakesh

AU - Muller, Karin H.

AU - Reid, David G.

AU - Skepper, Jeremy N.

AU - Wong, Wai Ching

AU - Brooks, Roger A.

AU - Green, Maggie

AU - Bihan, Dominique

AU - Farndale, Richard W.

AU - Slatter, David A.

AU - Shanahan, Catherine M.

AU - Duer, Melinda J.

PY - 2014/5/16

Y1 - 2014/5/16

N2 - Nuclear magnetic resonance (NMR) spectroscopy is useful to determine molecular structure in tissues grown in vitro only if their fidelity, relative to native tissue, can be established. Here, we use multidimensional NMR spectra of animal and in vitro model tissues as fingerprints of their respective molecular structures, allowing us to compare the intact tissues at atomic length scales. To obtain spectra from animal tissues, we developed a heavy mouse enriched by about 20% in the NMR-active isotopes carbon-13 and nitrogen-15. The resulting spectra allowed us to refine an in vitro model of developing bone and to probe its detailed structure. The identification of an unexpected molecule, poly(adenosine diphosphate ribose), that may be implicated in calcification of the bone matrix, illustrates the analytical power of this approach.

AB - Nuclear magnetic resonance (NMR) spectroscopy is useful to determine molecular structure in tissues grown in vitro only if their fidelity, relative to native tissue, can be established. Here, we use multidimensional NMR spectra of animal and in vitro model tissues as fingerprints of their respective molecular structures, allowing us to compare the intact tissues at atomic length scales. To obtain spectra from animal tissues, we developed a heavy mouse enriched by about 20% in the NMR-active isotopes carbon-13 and nitrogen-15. The resulting spectra allowed us to refine an in vitro model of developing bone and to probe its detailed structure. The identification of an unexpected molecule, poly(adenosine diphosphate ribose), that may be implicated in calcification of the bone matrix, illustrates the analytical power of this approach.

KW - SOLID-STATE NMR

KW - ORGANIC-MINERAL INTERFACE

KW - POLY(ADP-RIBOSE) POLYMERASE

KW - MAGNETIC-RESONANCE

KW - CHEMICAL-SHIFTS

KW - BONE

KW - C-13

KW - DYNAMICS

KW - SURFACE

KW - DEATH

U2 - 10.1126/science.1248167

DO - 10.1126/science.1248167

M3 - Article

SN - 0036-8075

VL - 344

SP - 742

EP - 746

JO - Science

JF - Science

IS - 6185

ER -