Non-haem iron transport in the rat proximal colon

K L Johnston, D M Johnson, J Marks, S K Srai, E S Debnam, P A Sharp

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Background Only 10% of dietary iron is absorbed in the duodenum which implies that 90% (approximately 9 mg day(-1)) reaches the lower small intestine and colon. Therefore the purpose of this study was to assess the iron transport capacity of the rat proximal colon and to determine whether iron absorption is regulated by changes in dietary iron content. Materials and methods Rats were fed for 14 days on either iron adequate (44 mg Fe kg(-1) diet) or iron-deficient (<0.5 mg Fe kg(-1) diet) diets. The Fe-59 transport across the colonic epithelium and its subsequent appearance in the blood were measured in vivo. In separate studies the colon was excised and used to measure divalent metal transporter expression. Results Divalent metal transporter (DMT1) was expressed at the apical membrane of the surface epithelium in rat proximal colon. In animals fed an iron-deficient diet, DMT1 mRNA and protein expression were increased. This was accompanied by a significant increase in tissue Fe-59 uptake. Conclusions The proximal colon can absorb non-haem iron from the intestinal lumen. The purpose of this mechanism remains to be elucidated
Original languageEnglish
Pages (from-to)35 - 40
Number of pages6
JournalEuropean Journal of Clinical Investigation
Volume36
Issue number1
DOIs
Publication statusPublished - Jan 2006

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