Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis

Nadia Chaher; Sara Lacerda; Giuseppe Digilio; Sergio Padovan; Ling Gao; Begona Lavin; Rachele Stefania; Carlos Velasco Jimeno; Gastão Cruz; Claudia Prieto Vasquez; Rene Botnar; Alkystis Phinikaridou

doi:10.1038/s44303-024-00037-z

Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis

Nadia Chaher, Sara Lacerda, Giuseppe Digilio, Sergio Padovan, Ling Gao, Begona Lavin, Rachele Stefania, Carlos Velasco Jimeno, Gastão Cruz, Claudia Prieto Vasquez, Rene Botnar, Alkystis Phinikaridou^*

^*Corresponding author for this work

Biomedical Engineering Department

Research output: Contribution to journal › Article › peer-review

Abstract

Heart failure (HF) affects 64 million people globally with enormous societal and healthcare costs. Myocardial fibrosis, characterised by changes in collagen content drives HF. Despite evidence that collagen type III (COL3) content changes during myocardial fibrosis, in vivo imaging of COL3 has not been achieved. Here, we discovered the first imaging probe that binds to COL3 with high affinity and specificity, by screening candidate peptide-based probes. Characterisation of the probe showed favourable magnetic and biodistribution properties. The probe’s potential for in vivo molecular cardiac magnetic resonance imaging was evaluated in a murine model of myocardial infarction. Using the new probe, we were able to map and quantify, previously undetectable, spatiotemporal changes in COL3 after myocardial infarction and monitor response to treatment. This innovative probe provides a promising tool to non-invasively study the unexplored roles of COL3 in cardiac fibrosis and other cardiovascular conditions marked by changes in COL3.

Original language	English
Journal	npj Imaging
DOIs	https://doi.org/10.1038/s44303-024-00037-z
Publication status	Published - 17 Sept 2024

Keywords

Molecular imaging
Magnetic resonance imaging
Chemical biology
Fibrosis
Imaging probes

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title = "Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis",

abstract = "Heart failure (HF) affects 64 million people globally with enormous societal and healthcare costs. Myocardial fibrosis, characterised by changes in collagen content drives HF. Despite evidence that collagen type III (COL3) content changes during myocardial fibrosis, in vivo imaging of COL3 has not been achieved. Here, we discovered the first imaging probe that binds to COL3 with high affinity and specificity, by screening candidate peptide-based probes. Characterisation of the probe showed favourable magnetic and biodistribution properties. The probe{\textquoteright}s potential for in vivo molecular cardiac magnetic resonance imaging was evaluated in a murine model of myocardial infarction. Using the new probe, we were able to map and quantify, previously undetectable, spatiotemporal changes in COL3 after myocardial infarction and monitor response to treatment. This innovative probe provides a promising tool to non-invasively study the unexplored roles of COL3 in cardiac fibrosis and other cardiovascular conditions marked by changes in COL3.",

keywords = "Molecular imaging, Magnetic resonance imaging, Chemical biology, Fibrosis, Imaging probes",

author = "Nadia Chaher and Sara Lacerda and Giuseppe Digilio and Sergio Padovan and Ling Gao and Begona Lavin and Rachele Stefania and {Velasco Jimeno}, Carlos and Gast{\~a}o Cruz and {Prieto Vasquez}, Claudia and Rene Botnar and Alkystis Phinikaridou",

year = "2024",

month = sep,

day = "17",

doi = "10.1038/s44303-024-00037-z",

language = "English",

journal = "npj Imaging",

issn = "2948-197X",

publisher = "Nature Publishing Group",

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T1 - Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis

AU - Chaher, Nadia

AU - Lacerda, Sara

AU - Digilio, Giuseppe

AU - Padovan, Sergio

AU - Gao, Ling

AU - Lavin, Begona

AU - Stefania, Rachele

AU - Velasco Jimeno, Carlos

AU - Cruz, Gastão

AU - Prieto Vasquez, Claudia

AU - Botnar, Rene

AU - Phinikaridou, Alkystis

PY - 2024/9/17

Y1 - 2024/9/17

N2 - Heart failure (HF) affects 64 million people globally with enormous societal and healthcare costs. Myocardial fibrosis, characterised by changes in collagen content drives HF. Despite evidence that collagen type III (COL3) content changes during myocardial fibrosis, in vivo imaging of COL3 has not been achieved. Here, we discovered the first imaging probe that binds to COL3 with high affinity and specificity, by screening candidate peptide-based probes. Characterisation of the probe showed favourable magnetic and biodistribution properties. The probe’s potential for in vivo molecular cardiac magnetic resonance imaging was evaluated in a murine model of myocardial infarction. Using the new probe, we were able to map and quantify, previously undetectable, spatiotemporal changes in COL3 after myocardial infarction and monitor response to treatment. This innovative probe provides a promising tool to non-invasively study the unexplored roles of COL3 in cardiac fibrosis and other cardiovascular conditions marked by changes in COL3.

AB - Heart failure (HF) affects 64 million people globally with enormous societal and healthcare costs. Myocardial fibrosis, characterised by changes in collagen content drives HF. Despite evidence that collagen type III (COL3) content changes during myocardial fibrosis, in vivo imaging of COL3 has not been achieved. Here, we discovered the first imaging probe that binds to COL3 with high affinity and specificity, by screening candidate peptide-based probes. Characterisation of the probe showed favourable magnetic and biodistribution properties. The probe’s potential for in vivo molecular cardiac magnetic resonance imaging was evaluated in a murine model of myocardial infarction. Using the new probe, we were able to map and quantify, previously undetectable, spatiotemporal changes in COL3 after myocardial infarction and monitor response to treatment. This innovative probe provides a promising tool to non-invasively study the unexplored roles of COL3 in cardiac fibrosis and other cardiovascular conditions marked by changes in COL3.

KW - Molecular imaging

KW - Magnetic resonance imaging

KW - Chemical biology

KW - Fibrosis

KW - Imaging probes

U2 - 10.1038/s44303-024-00037-z

DO - 10.1038/s44303-024-00037-z

M3 - Article

SN - 2948-197X

JO - npj Imaging

JF - npj Imaging

ER -

Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis

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Keywords

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