Noninvasive Assessment of Atherosclerotic Plaque Progression in ApoE(-/-) Mice Using Susceptibility Gradient Mapping

TY - JOUR

T1 - Noninvasive Assessment of Atherosclerotic Plaque Progression in ApoE(-/-) Mice Using Susceptibility Gradient Mapping

AU - Makowski, Marcus R.

AU - Varma, Gopal

AU - Wiethoff, Andrea J.

AU - Smith, Alberto

AU - Mattock, Katherine

AU - Jansen, Christian H. P.

AU - Warley, Alice

AU - Taupitz, Matthias

AU - Schaeffter, Tobias

AU - Botnar, Rene M.

PY - 2011/5

Y1 - 2011/5

N2 - Background-Macrophages have been identified as a major contributor to plaque development and destabilization in atherosclerosis. The aim of this study was to noninvasively assess uptake of citrate coated very small iron oxide particles at different stages of plaque development in the brachiocephalic artery of apoE(-/-) mice. Susceptibility gradient mapping (SGM) was applied to generate positive contrast images and to quantify iron oxide uptake. Methods and Results-ApoE(-/-) mice were fed a high-fat diet for 4, 8, or 12 weeks; 300 mu mol Fe/kg was injected 24 and 48 hours before final MRI. Increasing very small iron oxide particle uptake was observed over the course of atherosclerotic plaque development. Simultaneous administration of pravastatin led to a significant decrease in very small iron oxide particle uptake, assessed by mass spectroscopy and histology. SGM-MRI allowed the generation of positive contrast images, and magnitudes (mT/m) of contrast enhancement in SG parameter maps significantly correlated with the absolute iron oxide content (R-2=0.70, P

AB - Background-Macrophages have been identified as a major contributor to plaque development and destabilization in atherosclerosis. The aim of this study was to noninvasively assess uptake of citrate coated very small iron oxide particles at different stages of plaque development in the brachiocephalic artery of apoE(-/-) mice. Susceptibility gradient mapping (SGM) was applied to generate positive contrast images and to quantify iron oxide uptake. Methods and Results-ApoE(-/-) mice were fed a high-fat diet for 4, 8, or 12 weeks; 300 mu mol Fe/kg was injected 24 and 48 hours before final MRI. Increasing very small iron oxide particle uptake was observed over the course of atherosclerotic plaque development. Simultaneous administration of pravastatin led to a significant decrease in very small iron oxide particle uptake, assessed by mass spectroscopy and histology. SGM-MRI allowed the generation of positive contrast images, and magnitudes (mT/m) of contrast enhancement in SG parameter maps significantly correlated with the absolute iron oxide content (R-2=0.70, P

U2 - 10.1161/CIRCIMAGING.110.957209

DO - 10.1161/CIRCIMAGING.110.957209

M3 - Article

SN - 1942-0080

VL - 4

SP - 295 - U153

JO - Circulation-Cardiovascular imaging

JF - Circulation-Cardiovascular imaging

IS - 3

ER -