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Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development

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Swapna Thomas-Jinu, Patricia M Gordon, Triona Fielding, Richard Taylor, Bradley N Smith, Victoria Snowden, Eric Blanc, Caroline Vance, Simon Topp, Chun-Hao Wong, Holger Bielen, Kelly Williams, Emily P McCann, Garth A Nicholson, Alejandro Pan-Vazquez, Archa H Fox, Charles S Bond, William S Talbot, Ian P Blair, Christopher E Shaw & 1 more Corinne Houart

Original languageEnglish
Article numbere5
Pages (from-to)332-336
JournalNeuron
Volume94
Issue number2
Early online date6 Apr 2017
DOIs
Accepted/In press17 Mar 2017
E-pub ahead of print6 Apr 2017
Published19 Apr 2017

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Abstract

Recent progress revealed the complexity of RNA processing and its association to human disorders. Here, we unveil a new facet of this complexity. Complete loss of function of the ubiquitous splicing factor SFPQ affects zebrafish motoneuron differentiation cell autonomously. In addition to its nuclear localization, the protein unexpectedly localizes to motor axons. The cytosolic version of SFPQ abolishes motor axonal defects, rescuing key transcripts, and restores motility in the paralyzed sfpq null mutants, indicating a non-nuclear processing role in motor axons. Novel variants affecting the conserved coiled-coil domain, so far exclusively found in fALS exomes, specifically affect the ability of SFPQ to localize in axons. They broadly rescue morphology and motility in the zebrafish mutant, but alter motor axon morphology, demonstrating functional requirement for axonal SFPQ. Altogether, we uncover the axonal function of the splicing factor SFPQ in motor development and highlight the importance of the coiled-coil domain in this process.

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