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Notch Signaling Regulates Muscle Stem Cell Homeostasis and Regeneration in a Teleost Fish

Research output: Contribution to journalArticlepeer-review

Sami H.A. Sultan, Carlene Dyer, Robert D. Knight

Original languageEnglish
Article number726281
JournalFrontiers in Cell and Developmental Biology
Early online date28 Sep 2021
E-pub ahead of print28 Sep 2021
Published28 Sep 2021

Bibliographical note

Funding Information: The authors thank Oscar de Pena for critical reading of the article and the NC3Rs for constructive engagement in experiment design and interpretation. Funding Information: This work was supported by a NC3Rs funded Ph.D. studentship (NC/P002048/1) and a BBSRC project grant (BB/P002390/1) to RK. This research was also funded in part by the Wellcome Trust (101529/Z/13/Z). For the purpose of Open Access, the authors have applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. Publisher Copyright: © Copyright © 2021 Sultan, Dyer and Knight.

King's Authors


Muscle regeneration is mediated by the activity of resident muscle satellite cells (muSCs) that express Pax7. In mouse Notch signaling regulates muSCs during quiescence and promotes muSC proliferation in regeneration. It is unclear if these roles of Notch in regulating muSC biology are conserved across vertebrates or are a mammalian specific feature. We have therefore investigated the role of Notch in regulating muSC homeostasis and regeneration in a teleost fish, the zebrafish. We have also tested whether muSCs show differential sensitivity to Notch during myotome development. In an absence of injury Notch is important for preventing muSC proliferation at the vertical myoseptum. In contrast, Notch signaling promotes proliferation and prevents differentiation in the context of injury. Notch is required for the proliferative response to injury at early and later larval stages, suggesting it plays a similar role in regulating muSCs at developing and adult stages. Our results reveal a conserved role for Notch signaling in regulating muSCs under homeostasis and for promoting proliferation during regeneration in teleost fish.

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