Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis

Karmel Webb; Miguel Cámara; Nur Masirah M. Zain; Nigel Halliday; Kenneth D. Bruce; Edward F. Nash; Joanna L. Whitehouse; Alan Knox; Douglas Forrester; Alan R. Smyth; Paul Williams; Andrew Fogarty; Helen L. Barr

doi:10.1016/j.jcf.2021.08.030

Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis

Karmel Webb^*, Miguel Cámara, Nur Masirah M. Zain, Nigel Halliday, Kenneth D. Bruce, Edward F. Nash, Joanna L. Whitehouse, Alan Knox, Douglas Forrester, Alan R. Smyth, Paul Williams, Andrew Fogarty, Helen L. Barr

^*Corresponding author for this work

Institute of Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Pseudomonas aeruginosa produces specific signalling molecules, 2-alkyl-4-quinolones (AQs) that are detectable in the sputum of adults with cystic fibrosis (CF) and who have pulmonary infection with this opportunistic pathogen. This study aimed to determine whether AQs could be detected in saliva of patients with CF and known infection with Pseudomonas aeruginosa. Saliva and sputum samples were obtained from 89 adults with CF and analyzed using liquid chromatography-tandem mass spectrometry. AQs were detected in 39/89 (43.8%) saliva samples and 70/77(90.9%) sputum samples. Salivary AQs had a sensitivity of 50% (95%CI; 37.8; 62.2), specificity of 100% (95%CI; 47.8; 100), when compared to a molecular microbiological measure of P. aeruginosa in sputum as measured using polymerase chain reaction. Specific AQs produced by P. aeruginosa can be detected in the saliva and warrant investigation as potential non-invasive biomarkers of pulmonary P. aeruginosa.

Original language	English
Journal	Journal Of Cystic Fibrosis
DOIs	https://doi.org/10.1016/j.jcf.2021.08.030
Publication status	Accepted/In press - 2021

Keywords

Cystic fibrosis
Pseudomonas aeruginosa
Quorum sensing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jcf.2021.08.030

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Webb, K., Cámara, M., Zain, N. M. M., Halliday, N., Bruce, K. D., Nash, E. F., Whitehouse, J. L., Knox, A., Forrester, D., Smyth, A. R., Williams, P., Fogarty, A., & Barr, H. L. (Accepted/In press). Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis. Journal Of Cystic Fibrosis. https://doi.org/10.1016/j.jcf.2021.08.030

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title = "Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis",

abstract = "Pseudomonas aeruginosa produces specific signalling molecules, 2-alkyl-4-quinolones (AQs) that are detectable in the sputum of adults with cystic fibrosis (CF) and who have pulmonary infection with this opportunistic pathogen. This study aimed to determine whether AQs could be detected in saliva of patients with CF and known infection with Pseudomonas aeruginosa. Saliva and sputum samples were obtained from 89 adults with CF and analyzed using liquid chromatography-tandem mass spectrometry. AQs were detected in 39/89 (43.8%) saliva samples and 70/77(90.9%) sputum samples. Salivary AQs had a sensitivity of 50% (95%CI; 37.8; 62.2), specificity of 100% (95%CI; 47.8; 100), when compared to a molecular microbiological measure of P. aeruginosa in sputum as measured using polymerase chain reaction. Specific AQs produced by P. aeruginosa can be detected in the saliva and warrant investigation as potential non-invasive biomarkers of pulmonary P. aeruginosa.",

keywords = "Cystic fibrosis, Pseudomonas aeruginosa, Quorum sensing",

author = "Karmel Webb and Miguel C{\'a}mara and Zain, {Nur Masirah M.} and Nigel Halliday and Bruce, {Kenneth D.} and Nash, {Edward F.} and Whitehouse, {Joanna L.} and Alan Knox and Douglas Forrester and Smyth, {Alan R.} and Paul Williams and Andrew Fogarty and Barr, {Helen L.}",

note = "Funding Information: Funding contributions from Nottingham MRC Molecular Pathology Node, NIHR Nottingham Biomedical Research Centre, and the U.K. Medical Research Council (grant number G0801558/1). MC and PW are partly funded by the National Biofilms Innovation Centre (NBIC) which is an Innovation and Knowledge Centre funded by the Biotechnology and Biological Sciences Research Council, Innovate UK and the Hartree Centre (Award Number BB/R012415/1). Publisher Copyright: {\textcopyright} 2021 European Cystic Fibrosis Society Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

doi = "10.1016/j.jcf.2021.08.030",

language = "English",

journal = "Journal Of Cystic Fibrosis",

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Webb, K, Cámara, M, Zain, NMM, Halliday, N, Bruce, KD, Nash, EF, Whitehouse, JL, Knox, A, Forrester, D, Smyth, AR, Williams, P, Fogarty, A & Barr, HL 2021, 'Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis', Journal Of Cystic Fibrosis. https://doi.org/10.1016/j.jcf.2021.08.030

TY - JOUR

T1 - Novel detection of specific bacterial quorum sensing molecules in saliva

T2 - Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis

AU - Webb, Karmel

AU - Cámara, Miguel

AU - Zain, Nur Masirah M.

AU - Halliday, Nigel

AU - Bruce, Kenneth D.

AU - Nash, Edward F.

AU - Whitehouse, Joanna L.

AU - Knox, Alan

AU - Forrester, Douglas

AU - Smyth, Alan R.

AU - Williams, Paul

AU - Fogarty, Andrew

AU - Barr, Helen L.

N1 - Funding Information: Funding contributions from Nottingham MRC Molecular Pathology Node, NIHR Nottingham Biomedical Research Centre, and the U.K. Medical Research Council (grant number G0801558/1). MC and PW are partly funded by the National Biofilms Innovation Centre (NBIC) which is an Innovation and Knowledge Centre funded by the Biotechnology and Biological Sciences Research Council, Innovate UK and the Hartree Centre (Award Number BB/R012415/1). Publisher Copyright: © 2021 European Cystic Fibrosis Society Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021

Y1 - 2021

N2 - Pseudomonas aeruginosa produces specific signalling molecules, 2-alkyl-4-quinolones (AQs) that are detectable in the sputum of adults with cystic fibrosis (CF) and who have pulmonary infection with this opportunistic pathogen. This study aimed to determine whether AQs could be detected in saliva of patients with CF and known infection with Pseudomonas aeruginosa. Saliva and sputum samples were obtained from 89 adults with CF and analyzed using liquid chromatography-tandem mass spectrometry. AQs were detected in 39/89 (43.8%) saliva samples and 70/77(90.9%) sputum samples. Salivary AQs had a sensitivity of 50% (95%CI; 37.8; 62.2), specificity of 100% (95%CI; 47.8; 100), when compared to a molecular microbiological measure of P. aeruginosa in sputum as measured using polymerase chain reaction. Specific AQs produced by P. aeruginosa can be detected in the saliva and warrant investigation as potential non-invasive biomarkers of pulmonary P. aeruginosa.

AB - Pseudomonas aeruginosa produces specific signalling molecules, 2-alkyl-4-quinolones (AQs) that are detectable in the sputum of adults with cystic fibrosis (CF) and who have pulmonary infection with this opportunistic pathogen. This study aimed to determine whether AQs could be detected in saliva of patients with CF and known infection with Pseudomonas aeruginosa. Saliva and sputum samples were obtained from 89 adults with CF and analyzed using liquid chromatography-tandem mass spectrometry. AQs were detected in 39/89 (43.8%) saliva samples and 70/77(90.9%) sputum samples. Salivary AQs had a sensitivity of 50% (95%CI; 37.8; 62.2), specificity of 100% (95%CI; 47.8; 100), when compared to a molecular microbiological measure of P. aeruginosa in sputum as measured using polymerase chain reaction. Specific AQs produced by P. aeruginosa can be detected in the saliva and warrant investigation as potential non-invasive biomarkers of pulmonary P. aeruginosa.

KW - Cystic fibrosis

KW - Pseudomonas aeruginosa

KW - Quorum sensing

UR - http://www.scopus.com/inward/record.url?scp=85114719816&partnerID=8YFLogxK

U2 - 10.1016/j.jcf.2021.08.030

DO - 10.1016/j.jcf.2021.08.030

M3 - Article

AN - SCOPUS:85114719816

SN - 1569-1993

JO - Journal Of Cystic Fibrosis

JF - Journal Of Cystic Fibrosis

ER -

Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis

Abstract

Keywords

UN SDGs

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