Novel zwitterionic polyphosphorylcholine monolithic column for hydrophilic interaction chromatography

Zhengjin Jiang, John Reilly, Brian Everatt, Norman W. Smith

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

A novel porous zwitterionic monolith was prepared by thermal co-polymerisation of 2-methacryloyloxyethyl phosphorylcholine (MPC) and ethylene glycol dimethacrylate (EDMA) within 100 mu m I.D. capillaries. Mercury intrusion porosimetry, scanning electron microscopy (SEM), micro-HPLC (mu-HPLC), elemental analysis and zeta-potential analysis were used to evaluate the monolithic structure. No evidence of swelling or shrinking of the monolith in different polarity solvents was observed. A typical hydrophilic liquid chromatography(HILIC) mechanism was observed at high organic solvent content (acetonitrile >60%). The phosphorylcholine(PC) functionality has both a positively charged quaternary ammonium and a negatively charged phosphate group. For charged analytes, a weak electrostatic interaction was also observed by studying the influence of mobile phase pH and salt concentration on their retentions on the poly(MPC-co-EDMA) monolithic column. The optimised poly(MPC-co-EDMA) monolith showed very good selectivities for a range of polar test analytes, especially small peptides. This might be ascribed to the good biocompatibility of PC functionality. At low organic solvent content, baseline separation was also observed for a test mixture of seven alkylphenones by a reversed-phase separation mechanism. (c) 2009 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)2439 - 2448
Number of pages10
JournalJournal of Chromatography A
Volume1216
Issue number12
DOIs
Publication statusPublished - 20 Mar 2009

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