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Nox2 Is Required for Macrophage Chemotaxis towards CSF-1

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Nox2 Is Required for Macrophage Chemotaxis towards CSF-1. / Chaubey, Sanjay; Jones, Gareth E; Shah, Ajay M; Cave, Alison C; Wells, Claire M.

In: PL o S One , Vol. 8, No. 2, e54869, 01.02.2013.

Research output: Contribution to journalArticle

Harvard

Chaubey, S, Jones, GE, Shah, AM, Cave, AC & Wells, CM 2013, 'Nox2 Is Required for Macrophage Chemotaxis towards CSF-1', PL o S One , vol. 8, no. 2, e54869. https://doi.org/10.1371/journal.pone.0054869

APA

Chaubey, S., Jones, G. E., Shah, A. M., Cave, A. C., & Wells, C. M. (2013). Nox2 Is Required for Macrophage Chemotaxis towards CSF-1. PL o S One , 8(2), [e54869]. https://doi.org/10.1371/journal.pone.0054869

Vancouver

Chaubey S, Jones GE, Shah AM, Cave AC, Wells CM. Nox2 Is Required for Macrophage Chemotaxis towards CSF-1. PL o S One . 2013 Feb 1;8(2). e54869. https://doi.org/10.1371/journal.pone.0054869

Author

Chaubey, Sanjay ; Jones, Gareth E ; Shah, Ajay M ; Cave, Alison C ; Wells, Claire M. / Nox2 Is Required for Macrophage Chemotaxis towards CSF-1. In: PL o S One . 2013 ; Vol. 8, No. 2.

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@article{1e0682dbe0644cbf8b55e5e57221dc1f,
title = "Nox2 Is Required for Macrophage Chemotaxis towards CSF-1",
abstract = "Macrophage migration and infiltration is an important first step in many pathophysiological processes, in particular inflammatory diseases. Redox modulation of the migratory signalling processes has been reported in endothelial cells, vascular smooth muscle cells and fibroblasts. However the redox modulation of the migratory process in macrophages and in particular that from the NADPH oxidase-2 (Nox2) dependent ROS has not been established. To investigate the potential role of Nox2 in the migratory response of macrophages, bone marrow derived macrophages were obtained from WT and NOX2 knockout mice (Nox2KO) and subjected to CSF-1 stimulation. We report here that loss of Nox2 expression in BMM resulted in a significant reduction in the CSF-1 induced spreading response suggesting that Nox2 can modulate cytoskeletal events. Moreover, Nox2KO BMMs were deficient in cellular displacement in the presence of CSF-1. More significantly, when challenged with a gradient of CSF-1, Nox2KO BMMs showed a complete loss of chemotaxis accompanied by a reduction in cell migration speed and directional migration persistence. These results point to a specific role for Nox2KO downstream of CSF-1 during the BMM migratory response. Indeed, we have further found that Nox2KO BMMs display a significant reduction in the levels of ERK1/2 phosphorylation following stimulation with CSF-1.Thus Nox2 is important in BMM cellular motion to CSF-1 stimulation and necessary for their directed migration towards a CSF-1 gradient, highlighting Nox2 dependent signalling as a potential anti-inflammatory target.",
author = "Sanjay Chaubey and Jones, {Gareth E} and Shah, {Ajay M} and Cave, {Alison C} and Wells, {Claire M}",
year = "2013",
month = "2",
day = "1",
doi = "10.1371/journal.pone.0054869",
language = "English",
volume = "8",
journal = "PL o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Nox2 Is Required for Macrophage Chemotaxis towards CSF-1

AU - Chaubey, Sanjay

AU - Jones, Gareth E

AU - Shah, Ajay M

AU - Cave, Alison C

AU - Wells, Claire M

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Macrophage migration and infiltration is an important first step in many pathophysiological processes, in particular inflammatory diseases. Redox modulation of the migratory signalling processes has been reported in endothelial cells, vascular smooth muscle cells and fibroblasts. However the redox modulation of the migratory process in macrophages and in particular that from the NADPH oxidase-2 (Nox2) dependent ROS has not been established. To investigate the potential role of Nox2 in the migratory response of macrophages, bone marrow derived macrophages were obtained from WT and NOX2 knockout mice (Nox2KO) and subjected to CSF-1 stimulation. We report here that loss of Nox2 expression in BMM resulted in a significant reduction in the CSF-1 induced spreading response suggesting that Nox2 can modulate cytoskeletal events. Moreover, Nox2KO BMMs were deficient in cellular displacement in the presence of CSF-1. More significantly, when challenged with a gradient of CSF-1, Nox2KO BMMs showed a complete loss of chemotaxis accompanied by a reduction in cell migration speed and directional migration persistence. These results point to a specific role for Nox2KO downstream of CSF-1 during the BMM migratory response. Indeed, we have further found that Nox2KO BMMs display a significant reduction in the levels of ERK1/2 phosphorylation following stimulation with CSF-1.Thus Nox2 is important in BMM cellular motion to CSF-1 stimulation and necessary for their directed migration towards a CSF-1 gradient, highlighting Nox2 dependent signalling as a potential anti-inflammatory target.

AB - Macrophage migration and infiltration is an important first step in many pathophysiological processes, in particular inflammatory diseases. Redox modulation of the migratory signalling processes has been reported in endothelial cells, vascular smooth muscle cells and fibroblasts. However the redox modulation of the migratory process in macrophages and in particular that from the NADPH oxidase-2 (Nox2) dependent ROS has not been established. To investigate the potential role of Nox2 in the migratory response of macrophages, bone marrow derived macrophages were obtained from WT and NOX2 knockout mice (Nox2KO) and subjected to CSF-1 stimulation. We report here that loss of Nox2 expression in BMM resulted in a significant reduction in the CSF-1 induced spreading response suggesting that Nox2 can modulate cytoskeletal events. Moreover, Nox2KO BMMs were deficient in cellular displacement in the presence of CSF-1. More significantly, when challenged with a gradient of CSF-1, Nox2KO BMMs showed a complete loss of chemotaxis accompanied by a reduction in cell migration speed and directional migration persistence. These results point to a specific role for Nox2KO downstream of CSF-1 during the BMM migratory response. Indeed, we have further found that Nox2KO BMMs display a significant reduction in the levels of ERK1/2 phosphorylation following stimulation with CSF-1.Thus Nox2 is important in BMM cellular motion to CSF-1 stimulation and necessary for their directed migration towards a CSF-1 gradient, highlighting Nox2 dependent signalling as a potential anti-inflammatory target.

U2 - 10.1371/journal.pone.0054869

DO - 10.1371/journal.pone.0054869

M3 - Article

C2 - 23383302

VL - 8

JO - PL o S One

JF - PL o S One

SN - 1932-6203

IS - 2

M1 - e54869

ER -

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