Nucleic acid aptamers as aptasensors for plant biology

Sireethorn Tungsirisurp; Rachel O'Reilly; Richard Napier

doi:10.1016/j.tplants.2022.10.002

Nucleic acid aptamers as aptasensors for plant biology

Sireethorn Tungsirisurp^*, Rachel O'Reilly, Richard Napier^*

^*Corresponding author for this work

Analytical, Environmental & Forensic Sciences

Research output: Contribution to journal › Review article › peer-review

15 Citations (Scopus)

Abstract

Our knowledge of cell- and tissue-specific quantification of phytohormones is heavily reliant on laborious mass spectrometry techniques. Genetically encoded biosensors have allowed spatial and some temporal quantification of phytohormones intracellularly, but there is still limited information on their intercellular distributions. Here, we review nucleic acid aptamers as an emerging biosensing platform for the detection and quantification of analytes with high affinity and specificity. Options for DNA aptamer technology are explained through selection, sequencing analysis and techniques for evaluating affinity and specificity, and we focus on previously developed DNA aptamers against various plant analytes. We suggest how these tools might be applied in planta for quantification of molecules of interest both intracellularly and intercellularly.

Original language	English
Journal	TRENDS IN PLANT SCIENCE
DOIs	https://doi.org/10.1016/j.tplants.2022.10.002
Publication status	E-pub ahead of print - 7 Nov 2022

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10.1016/j.tplants.2022.10.002Licence: CC BY

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title = "Nucleic acid aptamers as aptasensors for plant biology",

abstract = "Our knowledge of cell- and tissue-specific quantification of phytohormones is heavily reliant on laborious mass spectrometry techniques. Genetically encoded biosensors have allowed spatial and some temporal quantification of phytohormones intracellularly, but there is still limited information on their intercellular distributions. Here, we review nucleic acid aptamers as an emerging biosensing platform for the detection and quantification of analytes with high affinity and specificity. Options for DNA aptamer technology are explained through selection, sequencing analysis and techniques for evaluating affinity and specificity, and we focus on previously developed DNA aptamers against various plant analytes. We suggest how these tools might be applied in planta for quantification of molecules of interest both intracellularly and intercellularly.",

author = "Sireethorn Tungsirisurp and Rachel O'Reilly and Richard Napier",

note = "Funding Information: The authors acknowledge financial support from the Leverhulme Trust (Grant number RPG-2016-452 ). Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

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doi = "10.1016/j.tplants.2022.10.002",

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AU - Tungsirisurp, Sireethorn

AU - O'Reilly, Rachel

AU - Napier, Richard

PY - 2022/11/7

Y1 - 2022/11/7

N2 - Our knowledge of cell- and tissue-specific quantification of phytohormones is heavily reliant on laborious mass spectrometry techniques. Genetically encoded biosensors have allowed spatial and some temporal quantification of phytohormones intracellularly, but there is still limited information on their intercellular distributions. Here, we review nucleic acid aptamers as an emerging biosensing platform for the detection and quantification of analytes with high affinity and specificity. Options for DNA aptamer technology are explained through selection, sequencing analysis and techniques for evaluating affinity and specificity, and we focus on previously developed DNA aptamers against various plant analytes. We suggest how these tools might be applied in planta for quantification of molecules of interest both intracellularly and intercellularly.

AB - Our knowledge of cell- and tissue-specific quantification of phytohormones is heavily reliant on laborious mass spectrometry techniques. Genetically encoded biosensors have allowed spatial and some temporal quantification of phytohormones intracellularly, but there is still limited information on their intercellular distributions. Here, we review nucleic acid aptamers as an emerging biosensing platform for the detection and quantification of analytes with high affinity and specificity. Options for DNA aptamer technology are explained through selection, sequencing analysis and techniques for evaluating affinity and specificity, and we focus on previously developed DNA aptamers against various plant analytes. We suggest how these tools might be applied in planta for quantification of molecules of interest both intracellularly and intercellularly.

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U2 - 10.1016/j.tplants.2022.10.002

DO - 10.1016/j.tplants.2022.10.002

M3 - Review article

SN - 1360-1385

JO - TRENDS IN PLANT SCIENCE

JF - TRENDS IN PLANT SCIENCE

ER -

Nucleic acid aptamers as aptasensors for plant biology

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