Nucleic Acids and Libraries

TY - PAT

T1 - Nucleic Acids and Libraries

AU - Gaken, Johannes

AU - Mohamedali, Azim

PY - 2008/10/16

Y1 - 2008/10/16

N2 - The invention relates to a nucleic acid comprising the following contiguous elements arranged in the 5 prime to 3 prime direction; a promoter; a selectable marker; a cloning site for receipt of a nucleic acid segment, said segment comprising a candidate miRNA target sequence; and a poly adenylation signal, said elements arranged such that a transcript directed by said promoter comprises said selectable marker, said candidate miRNA target sequence, and said poly adenylation signal in that order. Suitably the miRNA test sequence is or is derived from a 3'UTR. The invention also relates to methods for making and screening libraries.

AB - The invention relates to a nucleic acid comprising the following contiguous elements arranged in the 5 prime to 3 prime direction; a promoter; a selectable marker; a cloning site for receipt of a nucleic acid segment, said segment comprising a candidate miRNA target sequence; and a poly adenylation signal, said elements arranged such that a transcript directed by said promoter comprises said selectable marker, said candidate miRNA target sequence, and said poly adenylation signal in that order. Suitably the miRNA test sequence is or is derived from a 3'UTR. The invention also relates to methods for making and screening libraries.

KW - microRNA target discovery

M3 - Patent

M1 - WO2008122770

ER -